Screening of yeast co‐culture using crude hydrolysate for co‐fermentation of pentose and hexose

Juliana P. Sandri, Thais S. Milessi, Teresa C. Zangirolami, Solange I. Mussatto*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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The use of all sugars from biomass fractionation, i.e., hexoses and pentoses, is essential to develop feasible processes in a biorefinery context. Hexoses are converted into different products by most microorganisms, the main issue being the conversion of pentoses, especially when mixed with hexoses. Improving sugar conversion in co-fermentation is a major challenge that was explored in this work. Co-culture using S. cerevisiae and evolved K. marxianus strains was evaluated by a screening using 24 deep-well plates. Glucose depletion and 83% of xylose conversion were achieved from supplemented hemicellulosic hydrolysate, using 2/5 of deep-well volume filled with medium (at 150 rpm) and initial cell concentrations of 2.5 g/L for each yeast. The screening strategy provided reliable data that confirmed the co-culture as a promising approach for the co-assimilation of biomass-derived sugars. The importance of aeration control for effective xylose assimilation was also pointed out.
Original languageEnglish
JournalBiofuels, Bioproducts and Biorefining
Issue number6
Pages (from-to)1639-1653
Publication statusPublished - 2023


  • Yeast co-culture
  • Xylose conversion
  • Lignocellulosic biomass
  • Co-fermentation
  • Hydrolysate
  • Ethanol


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