Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases

Adiphol Dilokpimol; Bart Verkerk; Xinxin Li; Annie Bellemare; Mathieu Lavallee; Matthias Frommhagen; Emilie Nørmølle Underlin; Mirjam A. Kabel; Justin Powlowski; Adrian Tsang; Ronald P. de Vries

doi:10.1002/1873-3468.14322

Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases

Adiphol Dilokpimol
, Bart Verkerk
, Xinxin Li
, Annie Bellemare
, Mathieu Lavallee
, Matthias Frommhagen
, Emilie Nørmølle Underlin
, Mirjam A. Kabel
, Justin Powlowski
, Adrian Tsang
, Ronald P. de Vries^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Letter › peer-review

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Abstract

Feruloyl esterases (FAEs) and acetyl xylan esterases (AXEs) are important enzymes for plant biomass degradation and are both present in Carbohydrate Esterase family 1 (CE1) of the Carbohydrate-Active enZymes database. In this study, ten novel fungal CE1 enzymes from different subfamilies were heterologously produced and screened for their activity towards model and complex plant biomass substrates. CE1_1 enzymes possess AXE activity, while CE1_5 enzymes showed FAE activity. Two enzymes from CE1_2 and one from CE1_5 possess dual feruloyl/acetyl xylan esterase (FXE) activity, showing expansion of substrate specificity. The new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from feruloylated xylan, making them particularly interesting novel components of industrial enzyme cocktails for plant biomass degradation.

Original language	English
Journal	FEBS Letters
Volume	596
Issue number	15
Pages (from-to)	1932-1943
Number of pages	12
ISSN	0014-5793
DOIs	https://doi.org/10.1002/1873-3468.14322
Publication status	Published - 2022

Keywords

Feruloyl esterase
Acetyl xylan esterase
Hydroxycinnamic acid
Carbohydrate Esterase family 1
Plant biomass
Fungi

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title = "Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases",

abstract = "Feruloyl esterases (FAEs) and acetyl xylan esterases (AXEs) are important enzymes for plant biomass degradation and are both present in Carbohydrate Esterase family 1 (CE1) of the Carbohydrate-Active enZymes database. In this study, ten novel fungal CE1 enzymes from different subfamilies were heterologously produced and screened for their activity towards model and complex plant biomass substrates. CE1\_1 enzymes possess AXE activity, while CE1\_5 enzymes showed FAE activity. Two enzymes from CE1\_2 and one from CE1\_5 possess dual feruloyl/acetyl xylan esterase (FXE) activity, showing expansion of substrate specificity. The new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from feruloylated xylan, making them particularly interesting novel components of industrial enzyme cocktails for plant biomass degradation.",

keywords = "Feruloyl esterase, Acetyl xylan esterase, Hydroxycinnamic acid, Carbohydrate Esterase family 1, Plant biomass, Fungi",

author = "Adiphol Dilokpimol and Bart Verkerk and Xinxin Li and Annie Bellemare and Mathieu Lavallee and Matthias Frommhagen and Underlin, \{Emilie N{\o}rm{\o}lle\} and Kabel, \{Mirjam A.\} and Justin Powlowski and Adrian Tsang and \{de Vries\}, \{Ronald P.\}",

year = "2022",

doi = "10.1002/1873-3468.14322",

language = "English",

volume = "596",

pages = "1932--1943",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

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TY - JOUR

T1 - Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases

AU - Dilokpimol, Adiphol

AU - Verkerk, Bart

AU - Li, Xinxin

AU - Bellemare, Annie

AU - Lavallee, Mathieu

AU - Frommhagen, Matthias

AU - Underlin, Emilie Nørmølle

AU - Kabel, Mirjam A.

AU - Powlowski, Justin

AU - Tsang, Adrian

AU - de Vries, Ronald P.

PY - 2022

Y1 - 2022

N2 - Feruloyl esterases (FAEs) and acetyl xylan esterases (AXEs) are important enzymes for plant biomass degradation and are both present in Carbohydrate Esterase family 1 (CE1) of the Carbohydrate-Active enZymes database. In this study, ten novel fungal CE1 enzymes from different subfamilies were heterologously produced and screened for their activity towards model and complex plant biomass substrates. CE1_1 enzymes possess AXE activity, while CE1_5 enzymes showed FAE activity. Two enzymes from CE1_2 and one from CE1_5 possess dual feruloyl/acetyl xylan esterase (FXE) activity, showing expansion of substrate specificity. The new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from feruloylated xylan, making them particularly interesting novel components of industrial enzyme cocktails for plant biomass degradation.

AB - Feruloyl esterases (FAEs) and acetyl xylan esterases (AXEs) are important enzymes for plant biomass degradation and are both present in Carbohydrate Esterase family 1 (CE1) of the Carbohydrate-Active enZymes database. In this study, ten novel fungal CE1 enzymes from different subfamilies were heterologously produced and screened for their activity towards model and complex plant biomass substrates. CE1_1 enzymes possess AXE activity, while CE1_5 enzymes showed FAE activity. Two enzymes from CE1_2 and one from CE1_5 possess dual feruloyl/acetyl xylan esterase (FXE) activity, showing expansion of substrate specificity. The new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from feruloylated xylan, making them particularly interesting novel components of industrial enzyme cocktails for plant biomass degradation.

KW - Feruloyl esterase

KW - Acetyl xylan esterase

KW - Hydroxycinnamic acid

KW - Carbohydrate Esterase family 1

KW - Plant biomass

KW - Fungi

U2 - 10.1002/1873-3468.14322

DO - 10.1002/1873-3468.14322

M3 - Letter

C2 - 35187647

SN - 0014-5793

VL - 596

SP - 1932

EP - 1943

JO - FEBS Letters

JF - FEBS Letters

IS - 15

ER -

Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases

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Keywords

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