Screening of lactic acid bacteria for their potential as microbial cell factories for bioconversion of lignocellulosic feedstocks

Anna Monika Boguta; Francoise Bringel; Jan Martinussen; Peter Ruhdal Jensen

doi:10.1186/s12934-014-0097-0

Screening of lactic acid bacteria for their potential as microbial cell factories for bioconversion of lignocellulosic feedstocks

Anna Monika Boguta, Francoise Bringel, Jan Martinussen, Peter Ruhdal Jensen

National Food Institute

Université de Strasbourg

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Background: The use of fossil carbon sources for fuels and petrochemicals has serious impacts on our environment and is unable to meet the demand in the future. A promising and sustainable alternative is to substitute fossil carbon sources with microbial cell factories converting lignocellulosic biomass into desirable value added products. However, such bioprocesses require availability of suitable and efficient microbial biocatalysts, capable of utilizing C5 sugars and tolerant to inhibitory compounds generated during pretreatment of biomass. In this study, the performance of a collection of lactic acid bacteria was evaluated regarding their properties with respect to the conversion of lignocellulosic feedstocks. The strains were examined for their ability to utilize xylose and arabinose as well as their resistance towards common inhibitors from pretreated lignocellulosic biomass (furan derivatives, phenolic compounds, weak acids). Results: Among 296 tested Lactobacillus and Pediococcus strains, 3 L. pentosus, 1 P. acidilactici and 1 P. pentosaceus isolates were found to be both capable of utilizing xylose and arabinose and highly resistant to the key inhibitors from chemically pretreated lignocellulosic biomass. When tested in broth with commonly found combinations of inhibitors, the selected strains showed merely 4%, 1% and 37% drop in growth rates for sugarcane bagasse, wheat straw and soft wood representatives, respectively, as compared to Escherichia coli MG1655 showing decreased growth rates by 36%, 21% and 90%, respectively, under the same conditions. Conclusion: The study showed that some strains of Lactobacilli and Pediococci have the potential to be used as production platforms for value-added products from pretreated lignocellulosic biomass. Selected Lactobacilli and Pediococci strains were able to tolerate the key inhibitors in higher concentrations compared to E. coli; in addition, as these isolates were also capable of fermenting xylose and arabinose, they constitute good candidates for efficient lignocellulosic feedstock bioconversions.

Original language	English
Article number	97
Journal	Microbial Cell Factories
Volume	13
Issue number	1
Number of pages	16
ISSN	1475-2859
DOIs	https://doi.org/10.1186/s12934-014-0097-0
Publication status	Published - 2014

Bibliographical note

© 2014 Boguta et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain
Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article,
unless otherwise stated.

Keywords

sugarcane bagasse
wheat straw
Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Positive Cocci [07700] Pediococcus pentosaceus species Pediococcus acidilactici species
Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Regular Nonsporing Gram-Positive Rods [07830] Lactobacillus pentosus species fermentation agent
Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli species strain-MG1655
arabinose 147-81-9
C5 sugar
carbon 7440-44-0
furan 110-00-9
lignocellulosic
xylose 25990-60-7
10060, Biochemistry studies - General
10068, Biochemistry studies - Carbohydrates
31000, Physiology and biochemistry of bacteria
39008, Food microbiology - General and miscellaneous
fermentation method applied and field techniques
Biochemistry and Molecular Biophysics
Bioprocess Engineering
BIOTECHNOLOGY
ETHANOLOGENIC ESCHERICHIA-COLI
HEMICELLULOSE HYDROLYSATE
SACCHAROMYCES-CEREVISIAE
DEGRADATION-PRODUCTS
PICHIA-STIPITIS
WHEAT-STRAW
FERMENTATION
GROWTH
BIOMASS
INHIBITORS
Lactic acid bacteria
Fermentation inhibitors
Furfural
HMF
Lignocellulosic biomass
C5 sugars

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title = "Screening of lactic acid bacteria for their potential as microbial cell factories for bioconversion of lignocellulosic feedstocks",

abstract = "Background: The use of fossil carbon sources for fuels and petrochemicals has serious impacts on our environment and is unable to meet the demand in the future. A promising and sustainable alternative is to substitute fossil carbon sources with microbial cell factories converting lignocellulosic biomass into desirable value added products. However, such bioprocesses require availability of suitable and efficient microbial biocatalysts, capable of utilizing C5 sugars and tolerant to inhibitory compounds generated during pretreatment of biomass. In this study, the performance of a collection of lactic acid bacteria was evaluated regarding their properties with respect to the conversion of lignocellulosic feedstocks. The strains were examined for their ability to utilize xylose and arabinose as well as their resistance towards common inhibitors from pretreated lignocellulosic biomass (furan derivatives, phenolic compounds, weak acids). Results: Among 296 tested Lactobacillus and Pediococcus strains, 3 L. pentosus, 1 P. acidilactici and 1 P. pentosaceus isolates were found to be both capable of utilizing xylose and arabinose and highly resistant to the key inhibitors from chemically pretreated lignocellulosic biomass. When tested in broth with commonly found combinations of inhibitors, the selected strains showed merely 4%, 1% and 37% drop in growth rates for sugarcane bagasse, wheat straw and soft wood representatives, respectively, as compared to Escherichia coli MG1655 showing decreased growth rates by 36%, 21% and 90%, respectively, under the same conditions. Conclusion: The study showed that some strains of Lactobacilli and Pediococci have the potential to be used as production platforms for value-added products from pretreated lignocellulosic biomass. Selected Lactobacilli and Pediococci strains were able to tolerate the key inhibitors in higher concentrations compared to E. coli; in addition, as these isolates were also capable of fermenting xylose and arabinose, they constitute good candidates for efficient lignocellulosic feedstock bioconversions.",

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author = "Boguta, {Anna Monika} and Francoise Bringel and Jan Martinussen and Jensen, {Peter Ruhdal}",

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year = "2014",

doi = "10.1186/s12934-014-0097-0",

language = "English",

volume = "13",

journal = "Microbial Cell Factories",

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TY - JOUR

T1 - Screening of lactic acid bacteria for their potential as microbial cell factories for bioconversion of lignocellulosic feedstocks

AU - Boguta, Anna Monika

AU - Bringel, Francoise

AU - Martinussen, Jan

AU - Jensen, Peter Ruhdal

N1 - © 2014 Boguta et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PY - 2014

Y1 - 2014

N2 - Background: The use of fossil carbon sources for fuels and petrochemicals has serious impacts on our environment and is unable to meet the demand in the future. A promising and sustainable alternative is to substitute fossil carbon sources with microbial cell factories converting lignocellulosic biomass into desirable value added products. However, such bioprocesses require availability of suitable and efficient microbial biocatalysts, capable of utilizing C5 sugars and tolerant to inhibitory compounds generated during pretreatment of biomass. In this study, the performance of a collection of lactic acid bacteria was evaluated regarding their properties with respect to the conversion of lignocellulosic feedstocks. The strains were examined for their ability to utilize xylose and arabinose as well as their resistance towards common inhibitors from pretreated lignocellulosic biomass (furan derivatives, phenolic compounds, weak acids). Results: Among 296 tested Lactobacillus and Pediococcus strains, 3 L. pentosus, 1 P. acidilactici and 1 P. pentosaceus isolates were found to be both capable of utilizing xylose and arabinose and highly resistant to the key inhibitors from chemically pretreated lignocellulosic biomass. When tested in broth with commonly found combinations of inhibitors, the selected strains showed merely 4%, 1% and 37% drop in growth rates for sugarcane bagasse, wheat straw and soft wood representatives, respectively, as compared to Escherichia coli MG1655 showing decreased growth rates by 36%, 21% and 90%, respectively, under the same conditions. Conclusion: The study showed that some strains of Lactobacilli and Pediococci have the potential to be used as production platforms for value-added products from pretreated lignocellulosic biomass. Selected Lactobacilli and Pediococci strains were able to tolerate the key inhibitors in higher concentrations compared to E. coli; in addition, as these isolates were also capable of fermenting xylose and arabinose, they constitute good candidates for efficient lignocellulosic feedstock bioconversions.

AB - Background: The use of fossil carbon sources for fuels and petrochemicals has serious impacts on our environment and is unable to meet the demand in the future. A promising and sustainable alternative is to substitute fossil carbon sources with microbial cell factories converting lignocellulosic biomass into desirable value added products. However, such bioprocesses require availability of suitable and efficient microbial biocatalysts, capable of utilizing C5 sugars and tolerant to inhibitory compounds generated during pretreatment of biomass. In this study, the performance of a collection of lactic acid bacteria was evaluated regarding their properties with respect to the conversion of lignocellulosic feedstocks. The strains were examined for their ability to utilize xylose and arabinose as well as their resistance towards common inhibitors from pretreated lignocellulosic biomass (furan derivatives, phenolic compounds, weak acids). Results: Among 296 tested Lactobacillus and Pediococcus strains, 3 L. pentosus, 1 P. acidilactici and 1 P. pentosaceus isolates were found to be both capable of utilizing xylose and arabinose and highly resistant to the key inhibitors from chemically pretreated lignocellulosic biomass. When tested in broth with commonly found combinations of inhibitors, the selected strains showed merely 4%, 1% and 37% drop in growth rates for sugarcane bagasse, wheat straw and soft wood representatives, respectively, as compared to Escherichia coli MG1655 showing decreased growth rates by 36%, 21% and 90%, respectively, under the same conditions. Conclusion: The study showed that some strains of Lactobacilli and Pediococci have the potential to be used as production platforms for value-added products from pretreated lignocellulosic biomass. Selected Lactobacilli and Pediococci strains were able to tolerate the key inhibitors in higher concentrations compared to E. coli; in addition, as these isolates were also capable of fermenting xylose and arabinose, they constitute good candidates for efficient lignocellulosic feedstock bioconversions.

KW - sugarcane bagasse

KW - wheat straw

KW - Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Positive Cocci [07700] Pediococcus pentosaceus species Pediococcus acidilactici species

KW - Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Regular Nonsporing Gram-Positive Rods [07830] Lactobacillus pentosus species fermentation agent

KW - Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli species strain-MG1655

KW - arabinose 147-81-9

KW - C5 sugar

KW - carbon 7440-44-0

KW - furan 110-00-9

KW - lignocellulosic

KW - xylose 25990-60-7

KW - 10060, Biochemistry studies - General

KW - 10068, Biochemistry studies - Carbohydrates

KW - 31000, Physiology and biochemistry of bacteria

KW - 39008, Food microbiology - General and miscellaneous

KW - fermentation method applied and field techniques

KW - Biochemistry and Molecular Biophysics

KW - Bioprocess Engineering

KW - BIOTECHNOLOGY

KW - ETHANOLOGENIC ESCHERICHIA-COLI

KW - HEMICELLULOSE HYDROLYSATE

KW - SACCHAROMYCES-CEREVISIAE

KW - DEGRADATION-PRODUCTS

KW - PICHIA-STIPITIS

KW - WHEAT-STRAW

KW - FERMENTATION

KW - GROWTH

KW - BIOMASS

KW - INHIBITORS

KW - Lactic acid bacteria

KW - Fermentation inhibitors

KW - Furfural

KW - HMF

KW - Lignocellulosic biomass

KW - C5 sugars

U2 - 10.1186/s12934-014-0097-0

DO - 10.1186/s12934-014-0097-0

M3 - Journal article

C2 - 24997803

SN - 1475-2859

VL - 13

JO - Microbial Cell Factories

JF - Microbial Cell Factories

IS - 1

M1 - 97

ER -

Screening of lactic acid bacteria for their potential as microbial cell factories for bioconversion of lignocellulosic feedstocks

Abstract

Bibliographical note

Keywords

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