Screening of lactic acid bacteria for their potential as microbial cell factories for bioconversion of lignocellulosic feedstocks

Anna Monika Boguta, Francoise Bringel, Jan Martinussen, Peter Ruhdal Jensen

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Abstract

Background: The use of fossil carbon sources for fuels and petrochemicals has serious impacts on our environment and is unable to meet the demand in the future. A promising and sustainable alternative is to substitute fossil carbon sources with microbial cell factories converting lignocellulosic biomass into desirable value added products. However, such bioprocesses require availability of suitable and efficient microbial biocatalysts, capable of utilizing C5 sugars and tolerant to inhibitory compounds generated during pretreatment of biomass. In this study, the performance of a collection of lactic acid bacteria was evaluated regarding their properties with respect to the conversion of lignocellulosic feedstocks. The strains were examined for their ability to utilize xylose and arabinose as well as their resistance towards common inhibitors from pretreated lignocellulosic biomass (furan derivatives, phenolic compounds, weak acids). Results: Among 296 tested Lactobacillus and Pediococcus strains, 3 L. pentosus, 1 P. acidilactici and 1 P. pentosaceus isolates were found to be both capable of utilizing xylose and arabinose and highly resistant to the key inhibitors from chemically pretreated lignocellulosic biomass. When tested in broth with commonly found combinations of inhibitors, the selected strains showed merely 4%, 1% and 37% drop in growth rates for sugarcane bagasse, wheat straw and soft wood representatives, respectively, as compared to Escherichia coli MG1655 showing decreased growth rates by 36%, 21% and 90%, respectively, under the same conditions. Conclusion: The study showed that some strains of Lactobacilli and Pediococci have the potential to be used as production platforms for value-added products from pretreated lignocellulosic biomass. Selected Lactobacilli and Pediococci strains were able to tolerate the key inhibitors in higher concentrations compared to E. coli; in addition, as these isolates were also capable of fermenting xylose and arabinose, they constitute good candidates for efficient lignocellulosic feedstock bioconversions.
Original languageEnglish
Article number97
JournalMicrobial Cell Factories
Volume13
Issue number1
Number of pages16
ISSN1475-2859
DOIs
Publication statusPublished - 2014

Bibliographical note

© 2014 Boguta et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain
Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article,
unless otherwise stated.

Keywords

  • sugarcane bagasse
  • wheat straw
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Positive Cocci [07700] Pediococcus pentosaceus species Pediococcus acidilactici species
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Regular Nonsporing Gram-Positive Rods [07830] Lactobacillus pentosus species fermentation agent
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli species strain-MG1655
  • arabinose 147-81-9
  • C5 sugar
  • carbon 7440-44-0
  • furan 110-00-9
  • lignocellulosic
  • xylose 25990-60-7
  • 10060, Biochemistry studies - General
  • 10068, Biochemistry studies - Carbohydrates
  • 31000, Physiology and biochemistry of bacteria
  • 39008, Food microbiology - General and miscellaneous
  • fermentation method applied and field techniques
  • Biochemistry and Molecular Biophysics
  • Bioprocess Engineering
  • BIOTECHNOLOGY
  • ETHANOLOGENIC ESCHERICHIA-COLI
  • HEMICELLULOSE HYDROLYSATE
  • SACCHAROMYCES-CEREVISIAE
  • DEGRADATION-PRODUCTS
  • PICHIA-STIPITIS
  • WHEAT-STRAW
  • FERMENTATION
  • GROWTH
  • BIOMASS
  • INHIBITORS
  • Lactic acid bacteria
  • Fermentation inhibitors
  • Furfural
  • HMF
  • Lignocellulosic biomass
  • C5 sugars

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