Screen-Printed All-Polymer Aptasensor for Impedance Based Detection of Influenza A Virus

Julie Kirkegaard, Noemi Rozlosnik

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

    Abstract

    In this chapter a detailed description of the fabrication and testing of an aptasensor for influenza A virus detection is given. The sensor chip is an all-polymer chip fabricated with screen-printed poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) electrodes. Chip substrates are made by CO2 laser cutting of Poly(methyl methacrylate) (PMMA) sheets. Influenza A virus specific aptamers are immobilized onto the electrodes by UV cross-linking. Impedance based measurements at a single frequency, measured over time, are used to detect the virus in a buffer solution.
    Original languageEnglish
    Title of host publicationBiosensors and Biodetection : Methods and Protocols, Volume 2
    Number of pages16
    Volume1572
    PublisherSpringer
    Publication date2017
    Pages55-70
    Chapter5
    ISBN (Print)978-1-4939-6910-4
    ISBN (Electronic)978-1-4939-6911-1
    DOIs
    Publication statusPublished - 2017
    SeriesMethods in Molecular Biology
    ISSN1064-3745

    Keywords

    • Aptamers
    • Aptasensor
    • Impedance
    • Influenza A virus
    • PEDOT:PSS
    • Screen printing

    Cite this

    Kirkegaard, J., & Rozlosnik, N. (2017). Screen-Printed All-Polymer Aptasensor for Impedance Based Detection of Influenza A Virus. In Biosensors and Biodetection: Methods and Protocols, Volume 2 (Vol. 1572, pp. 55-70). Springer. Methods in Molecular Biology https://doi.org/10.1007/978-1-4939-6911-1_5