Sample preparation by cell guiding using negative dielectrophoresis

Troels Balmer Christensen, Christian Møller Pedersen, Dang Duong Bang, Anders Wolff

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    In this study, we present a microsystem designed for performing and testing dielectrophoretic (DEP) guiding of biological cells. Baker's yeast (Saccharomyces cerevisiae) is used as a model organism to study cell guiding in the system. The guiding efficiency as a function of flowrate is investigated and a decreased efficiency with increased flowrate is observed. In addition, the DEP behaviour of the yeast cells at different medium conductivities and applied frequencies is investigated. The chip is easily fabricated in a two-step process: Standard UV lithography techniques are used for electrode fabrication and laser ablation for channel structuring. The channel is seated with an indiumtinoxide (ITO) covered polymethylmethacrylate (PMMA) lid. The ITO serves as a transparent counter electrode. This conducting and transparent lid eliminates difficult top/bottom electrode alignment and gives visual access to the microchannel. (c) 2007 Elsevier B.V. All rights reserved.
    Original languageEnglish
    JournalMicroelectronic Engineering
    Volume84
    Issue number5-8
    Pages (from-to)1690-1693
    ISSN0167-9317
    DOIs
    Publication statusPublished - 2007

    Keywords

    • dielectrophoresis
    • cell guiding
    • sample pre-treatment
    • lab on a chip

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