Same-day PCR testing of Salmonella in meat: from research to routine application at slaughterhouses

Jeffrey Hoorfar, Charlotta Löfström, Mathilde Hartmann Josefsen, F. Hansen, S. Mansdal, J. Andersen, G. Pedersen

Research output: Contribution to journalJournal articleResearch

Abstract

Development of a rapid PCR technique is described, which enables slaughterhouses to apply same-day testing for Salmonella in carcasses and fresh meat. The technique is based on a shortened pre-enrichment time and 1-h DNA purification using paramagnetic beads (or an easy-to-use boiling method) followed by Salmonella detection by real-time PCR. Final protocols have been approved for meat testing (fresh meat and carcass swabs) by the Nordval validation organization for Nordic countries.
Original languageEnglish
JournalNew Food
Volume14
Issue number3
Pages (from-to)17-20
ISSN1461-4642
Publication statusPublished - 2011

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