Same-day PCR testing of Salmonella in meat: from research to routine application at slaughterhouses

Jeffrey Hoorfar; Charlotta Löfström; Mathilde Hartmann Josefsen; F. Hansen; S. Mansdal; J. Andersen; G. Pedersen

Same-day PCR testing of Salmonella in meat: from research to routine application at slaughterhouses

Jeffrey Hoorfar, Charlotta Löfström, Mathilde Hartmann Josefsen, F. Hansen, S. Mansdal, J. Andersen, G. Pedersen

National Food Institute

Research output: Contribution to journal › Journal article › Research

Abstract

Development of a rapid PCR technique is described, which enables slaughterhouses to apply same-day testing for Salmonella in carcasses and fresh meat. The technique is based on a shortened pre-enrichment time and 1-h DNA purification using paramagnetic beads (or an easy-to-use boiling method) followed by Salmonella detection by real-time PCR. Final protocols have been approved for meat testing (fresh meat and carcass swabs) by the Nordval validation organization for Nordic countries.

Original language	English
Journal	New Food
Volume	14
Issue number	3
Pages (from-to)	17-20
ISSN	1461-4642
Publication status	Published - 2011

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title = "Same-day PCR testing of Salmonella in meat: from research to routine application at slaughterhouses",

abstract = "Development of a rapid PCR technique is described, which enables slaughterhouses to apply same-day testing for Salmonella in carcasses and fresh meat. The technique is based on a shortened pre-enrichment time and 1-h DNA purification using paramagnetic beads (or an easy-to-use boiling method) followed by Salmonella detection by real-time PCR. Final protocols have been approved for meat testing (fresh meat and carcass swabs) by the Nordval validation organization for Nordic countries.",

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AU - Hoorfar, Jeffrey

AU - Löfström, Charlotta

AU - Josefsen, Mathilde Hartmann

AU - Hansen, F.

AU - Mansdal, S.

AU - Andersen, J.

AU - Pedersen, G.

PY - 2011

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AB - Development of a rapid PCR technique is described, which enables slaughterhouses to apply same-day testing for Salmonella in carcasses and fresh meat. The technique is based on a shortened pre-enrichment time and 1-h DNA purification using paramagnetic beads (or an easy-to-use boiling method) followed by Salmonella detection by real-time PCR. Final protocols have been approved for meat testing (fresh meat and carcass swabs) by the Nordval validation organization for Nordic countries.

M3 - Journal article

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VL - 14

SP - 17

EP - 20

JO - New Food

JF - New Food

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Same-day PCR testing of Salmonella in meat: from research to routine application at slaughterhouses

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