S. sanguinis adhesion on rough titanium surfaces: Effect of culture media

Ana G. Rodriguez-Hernandez, Jose A. Munoz-Tabares, Maria Godoy Gallardo, Antonio Juarez, Francisco-Javier Gil

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Bacterial colonization plays a key role in dental implant failure, because they attach directly on implant surface upon implantation. Between different types of bacteria associated with the oral environment, Streptococcus sanguinis is essential in this process since it is an early colonizer. In this work the relationship between titanium surfaces modified by shot blasting treatment and S. sanguinis adhesion; have been studied in approached human mouth environment. Bacteria pre-inoculated with routinaiy solution were put in contact with titanium samples, shot-blasted with alumina and silicon carbide, and adhesion results were compared with those obtained when bacteria were pre-inoculated with modified artificial saliva medium and on saliva pre-coated titanium samples. Our results showed that bacterial adhesion on titanium samples was influenced by culture conditions. When S. sanguinis was inoculated in routinary culture media, colonies forming unities per square millimeter presented an increment correlated with roughness and surface energy, but separated by the type of particle used during shot-blasting treatment; whereas in modified artificial saliva only a relationship between bacteria adhered and the increment in both roughness and surface energy were observed, regardless of the particle type. Finally, on human saliva pre-coated samples no significant differences were observed among roughness, surface energy or particle. (C) 2012 Elsevier B.V. All rights reserved.
Original languageEnglish
JournalMaterials Science and Engineering C: Materials for Biological Applications
Volume33
Issue number2
Pages (from-to)714-720
Number of pages7
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • S. sanguinis
  • Bacterial Adhesion
  • Titanium
  • Artificial saliva
  • Surface energy
  • Roughness

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