Rumen bacterial community transition during adaptation to high-grain diet

K. Tajima, S. Arai, K. Ogata, T. Nagamine, H. Matsui, M. Nakamura, Rustam Aminov, Y. Benno

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Abstract

Transitional changes of the ruminal bacterial community structure in cows during the switch from roughage to high-grain diet were monitored by PCR amplification and sequencing of 16S rDNA clone libraries. In total, one hundred fifty 16S rDNA sequences of almost full-length (1.4 kb) were analysed from three libraries prepared from the rumen fluid on day 0, 3, and 28 of switch to high-grain diet. In the first library (day 0, hay diet) of 51clones, 90.2% of sequences were belonging to the low G+C Gram-positive bacteria (LGCGPB) phylum, with the minor inclusion of the Cytophaga-Flavobacter-Bacteroides (CFB;3.9%), Proteobacteria (3.9%) and high G+C Gram-positive bacteria (HGCGPB; 2.0%) phyla-related sequences. Six LGCGPB sequences were clustered with the well-known cellulolytics of the rumen, Ruminococcus flavefaciens and R. albus. In the second library (day 3 of high-grain diet) of 58 clones, the LGCGPB-related sequences still dominated (72.4%), albeit being represented by other species than in the first library In particular, this library was enriched by representatives of Selenomonas-Succiniclasticum-Megasphaera group IX (17.2%), lactobacilli (6.9%) and Butyrivibrio fibrisolvens lineage 3-related (8.6%) sequences. Other phyla were represented by CFB (22.4%) and HGCGPB (3.4%). In the third library (day 28 of high-grain diet) of 41 clones, 95% of sequences fell into the LGCGPB phylum. About half of them (46%) were clustered within the Selenomonas-Succiniclasficum-Megasphaera group in Clostridium cluster TX. No HGCGPB-related sequences were detected and CFB was represented by only a single clone. No Streptococcus bovis-related sequences were detected in any of the three clone libraries. (C) 2000 Academic Press.
Original languageEnglish
JournalAnaerobe
Volume6
Issue number5
Pages (from-to)273-284
Number of pages12
ISSN1075-9964
DOIs
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • ribosome DNA
  • adaptation
  • animal experiment
  • article
  • Bacteroides
  • controlled study
  • dietary intake
  • DNA sequence
  • grain
  • Gram positive bacterium
  • Lactobacillus
  • nonhuman
  • phylogeny
  • polymerase chain reaction
  • priority journal
  • rumen bacteria
  • Ruminococcus
  • Acidaminococcaceae
  • Actinobacteria (class)
  • Animalia
  • Bacteria (microorganisms)
  • Butyrivibrio fibrisolvens
  • Clostridium
  • Cytophaga
  • Firmicutes
  • Flavobacter
  • Megasphaera
  • Posibacteria
  • Proteobacteria
  • Ruminococcus albus
  • Ruminococcus flavefaciens
  • Selenomonas
  • Streptococcus bovis
  • Succiniclasticum
  • 16S rDNA library
  • Community structure
  • Metabolic acidosis
  • Phylogenetic analysis
  • Metabolic acidosis, 16S rDNA library, phylogenetic analysis, community structure
  • MICROBIOLOGY
  • SEQUENCE-ANALYSIS
  • ACIDOSIS
  • DNA
  • metabolic acidosis
  • phylogenetic analysis
  • community structure
  • cellulolytics
  • G-C content
  • high-grain diet
  • high-grain diet adaptation
  • roughage
  • umen bacterial community transition
  • Actinomycetes and Related Organisms Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Irregular Nonsporing Gram-Positive Rods [08890] Butyrivibrio fibrisolvens
  • Artiodactyla Mammalia Vertebrata Chordata Animalia (Animals, Artiodactyls, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Vertebrates) - Bovidae [85715] cows
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Endospore-forming Gram-Positives [07810] Clostridium cluster IX
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Gram-Positive Cocci [07700] Ruminococcus albus Ruminococcus flavefaciens Streptococcus bovis
  • Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Regular Nonsporing Gram-Positive Rods [07830] lactobacilli
  • Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] Cytophaga-Flavobacter-Bacteroides Gram-positive bacteria Proteobacteria Selenomonas-Succiniclasticum-Megasphaera group IX bacteria rumen
  • 16S rDNA
  • 16S rDNA clone libraries
  • 03502, Genetics - General
  • 03506, Genetics - Animal
  • 07502, Ecology: environmental biology - General and methods
  • 07508, Ecology: environmental biology - Animal
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 14004, Digestive system - Physiology and biochemistry
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • Biochemistry and Molecular Biophysics
  • Environmental Sciences
  • rumen digestive system
  • rumen fluid digestive system
  • PCR amplification polymerase chain reaction amplification DNA amplification method
  • Ecology
  • Infection
  • Molecular Genetics

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