TY - JOUR
T1 - Round-robin comparison of methods for the detection of human enteric viruses in lettuce
AU - Le Guyader, Francoise S.
AU - Schultz, Anna Charlotte
AU - Haugarreau, Larissa
AU - Croci, Luciana
AU - Maunula, Leena
AU - Duizer, Erwin
AU - Lodder-Verschoor, Froukje
AU - Von Bonsdorff, Carl-Henrik
AU - Suffredini, Elizabetha
AU - van der Poel, Wim M. M.
AU - Reymundo, Rosanna
AU - Koopmans, Marion
PY - 2004
Y1 - 2004
N2 - Five methods that detect human enteric virus contamination in lettuce were compared. To mimic multiple contaminations as observed after sewage contamination, artificial contamination was with human calicivirus and poliovirus and animal calicivirus strains at different concentrations. Nucleic acid extractions were done at the same time in the same laboratory to reduce assay-to-assay variability. Results showed that the two critical steps are the washing step and removal of inhibitors. The more reliable methods (sensitivity, simplicity, low cost) included an elution/concentration step and a commercial kit. Such development of sensitive methods for viral detection in foods other than shellfish is important to improve food safety.
AB - Five methods that detect human enteric virus contamination in lettuce were compared. To mimic multiple contaminations as observed after sewage contamination, artificial contamination was with human calicivirus and poliovirus and animal calicivirus strains at different concentrations. Nucleic acid extractions were done at the same time in the same laboratory to reduce assay-to-assay variability. Results showed that the two critical steps are the washing step and removal of inhibitors. The more reliable methods (sensitivity, simplicity, low cost) included an elution/concentration step and a commercial kit. Such development of sensitive methods for viral detection in foods other than shellfish is important to improve food safety.
M3 - Journal article
SN - 0362-028X
VL - 67
SP - 2315
EP - 2319
JO - Journal of Food Protection
JF - Journal of Food Protection
IS - 10
ER -