Room-temperature solid phase ionic liquid (RTSPIL) coated Ω-transaminases: Development and application in organic solvents

B. Grabner; M. A. Nazario; M. T. Gundersen; S. Loïs; S. Fantini; S. Bartsch; J. M. Woodley; H. Gruber-Woelfler

doi:10.1016/j.mcat.2018.03.012

Room-temperature solid phase ionic liquid (RTSPIL) coated Ω-transaminases: Development and application in organic solvents

B. Grabner^*, M. A. Nazario, M. T. Gundersen, S. Loïs, S. Fantini, S. Bartsch, J. M. Woodley, H. Gruber-Woelfler

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co‐lyophilization, and showed increased stability in all of the five tested organic solvents. Co‐lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8‐fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5′‐phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5‐bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.

Original language	English
Journal	Molecular Catalysis
Volume	452
Pages (from-to)	11-19
ISSN	2468-8231
DOIs	https://doi.org/10.1016/j.mcat.2018.03.012
Publication status	Published - 2018

Keywords

Biocatalysis
Ionic liquid
Organic solvent
RTSPIL
Transaminase

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title = "Room-temperature solid phase ionic liquid (RTSPIL) coated Ω-transaminases: Development and application in organic solvents",

abstract = "ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co‐lyophilization, and showed increased stability in all of the five tested organic solvents. Co‐lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8‐fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5′‐phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5‐bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.",

keywords = "Biocatalysis, Ionic liquid, Organic solvent, RTSPIL, Transaminase",

author = "B. Grabner and Nazario, {M. A.} and Gundersen, {M. T.} and S. Lo{\"i}s and S. Fantini and S. Bartsch and Woodley, {J. M.} and H. Gruber-Woelfler",

year = "2018",

doi = "10.1016/j.mcat.2018.03.012",

language = "English",

volume = "452",

pages = "11--19",

journal = "Molecular Catalysis",

issn = "2468-8231",

publisher = "Elsevier",

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TY - JOUR

T1 - Room-temperature solid phase ionic liquid (RTSPIL) coated Ω-transaminases: Development and application in organic solvents

AU - Grabner, B.

AU - Nazario, M. A.

AU - Gundersen, M. T.

AU - Loïs, S.

AU - Fantini, S.

AU - Bartsch, S.

AU - Woodley, J. M.

AU - Gruber-Woelfler, H.

PY - 2018

Y1 - 2018

N2 - ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co‐lyophilization, and showed increased stability in all of the five tested organic solvents. Co‐lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8‐fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5′‐phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5‐bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.

AB - ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co‐lyophilization, and showed increased stability in all of the five tested organic solvents. Co‐lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8‐fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5′‐phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5‐bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.

KW - Biocatalysis

KW - Ionic liquid

KW - Organic solvent

KW - RTSPIL

KW - Transaminase

U2 - 10.1016/j.mcat.2018.03.012

DO - 10.1016/j.mcat.2018.03.012

M3 - Journal article

SN - 2468-8231

VL - 452

SP - 11

EP - 19

JO - Molecular Catalysis

JF - Molecular Catalysis

ER -

Room-temperature solid phase ionic liquid (RTSPIL) coated Ω-transaminases: Development and application in organic solvents

Abstract

Keywords

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