Robust High Performance Aquaporin based Biomimetic Membranes

Claus Helix Nielsen, Yichun Zhao, C. Qiu, S. Mentzel, J. Torres, T. Chuyang, Oliver Geschke, A. G. Fane

Research output: Contribution to journalConference abstract in journalResearchpeer-review

Abstract

Aquaporins are water channel proteins with high water permeability and solute rejection, which makes them promising for preparing high-performance biomimetic membranes. Despite the growing interest in aquaporin-based biomimetic membranes (ABMs), it is challenging to produce robust and defect-free ABMs that can be easily scaled up. We have constructed robust thin film composite (TFC) ABMs with surface areas up to 600 cm2 prepared by interfacial polymerization where Aquaporin Z-containing proteoliposomes were added to a m-phenylene-diamine aqueous solution thereby creating a polymer-rich film on top of a support membrane. Control membranes, either without aquaporins or with the inactive AqpZ R189A mutant aquaporin served as controls. The separation performance of the membranes was evaluated by cross-flow forward osmosis (FO) and reverse osmosis (RO) tests. In RO the ABM achieved a water permeability of ~ 4 L/(m2 h bar) with a NaCl rejection > 97% at an applied hydraulic pressure of 5 bar. The water permeability was ~40% higher compared to a commercial brackish water RO membrane (BW30) and an order of magnitude higher compared to a seawater RO membrane (SW30HR). In FO, the ABMs had > 90% rejection for urea and a water permeability around 10 L/(m2h) with 2M NaCl as draw solution. Our results demonstrate the feasibility of using aquaporin proteins in biomimetic membranes for technological applications.

Original languageEnglish
JournalBiophysical Journal
Volume104
Issue number2; SUPP/1
Pages (from-to)687a
ISSN0006-3495
Publication statusPublished - 2013

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