Robust biodegradation of naproxen and diclofenac by laccase immobilized using electrospun nanofibers with enhanced stability and reusability

Jakub Zdarta, Katarzyna Jankowska, Marta Wyszowska, Ewa Kijeńska-Gawrońska, Agnieszka Zgoła-Grześkowiak, Manuel Pinelo, Anne S. Meyer, Dariusz Moszyński, Teofil Jesionowski*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Enzymatic biodegradation of pharmaceuticals, using enzymes such as laccase, is a green solution for the removal of toxic pollutants that has attracted growing interest over recent years. Moreover, the application of immobilized biocatalysts is relevant for industrial applications, due to the improved stability and reusability of the immobilized enzymes. Thus, in the present study, laccase was immobilized by adsorption and encapsulation using poly(l-lactic acid)-co-poly(ε-caprolactone) (PLCL) electrospun nanofibers as a tailor-made support. The produced biocatalytic systems were applied in the biodegradation of two commonly used anti-inflammatories, naproxen and diclofenac, which are present in wastewaters at environmentally relevant concentrations. The results showed that under optimal process conditions (temperature 25 °C, pH 5 and 3 for naproxen and diclofenac respectively), even from a solution at a concentration of 1 mg L−1, over 90% of both pharmaceuticals was removed by encapsulated laccase in batch mode. Both immobilized enzymes also exhibited high reusability: after five reaction cycles approximately 60% and 40% of naproxen and diclofenac were removed by encapsulated and adsorbed laccase respectively. In addition, a thorough analysis was made of the products of biodegradation of the two studied pollutants. Furthermore, toxicity study of the mixture after biodegradation of the pharmaceuticals showed that the solutions obtained after the process were approximately 65% less toxic than the initial naproxen and diclofenac solutions.
Original languageEnglish
Article number109789
JournalMaterials Science and Engineering C: Biomimetic Materials, Sensors and Systems
Volume103
Number of pages10
ISSN0928-4931
DOIs
Publication statusPublished - 2019

Keywords

  • Laccase
  • Immobilization
  • Electrospun materials
  • Environmental pollutants
  • Pharmaceuticals
  • Biodegradation

Cite this

Zdarta, Jakub ; Jankowska, Katarzyna ; Wyszowska, Marta ; Kijeńska-Gawrońska, Ewa ; Zgoła-Grześkowiak, Agnieszka ; Pinelo, Manuel ; Meyer, Anne S. ; Moszyński, Dariusz ; Jesionowski, Teofil. / Robust biodegradation of naproxen and diclofenac by laccase immobilized using electrospun nanofibers with enhanced stability and reusability. In: Materials Science and Engineering C: Biomimetic Materials, Sensors and Systems. 2019 ; Vol. 103.
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abstract = "Enzymatic biodegradation of pharmaceuticals, using enzymes such as laccase, is a green solution for the removal of toxic pollutants that has attracted growing interest over recent years. Moreover, the application of immobilized biocatalysts is relevant for industrial applications, due to the improved stability and reusability of the immobilized enzymes. Thus, in the present study, laccase was immobilized by adsorption and encapsulation using poly(l-lactic acid)-co-poly(ε-caprolactone) (PLCL) electrospun nanofibers as a tailor-made support. The produced biocatalytic systems were applied in the biodegradation of two commonly used anti-inflammatories, naproxen and diclofenac, which are present in wastewaters at environmentally relevant concentrations. The results showed that under optimal process conditions (temperature 25 °C, pH 5 and 3 for naproxen and diclofenac respectively), even from a solution at a concentration of 1 mg L−1, over 90{\%} of both pharmaceuticals was removed by encapsulated laccase in batch mode. Both immobilized enzymes also exhibited high reusability: after five reaction cycles approximately 60{\%} and 40{\%} of naproxen and diclofenac were removed by encapsulated and adsorbed laccase respectively. In addition, a thorough analysis was made of the products of biodegradation of the two studied pollutants. Furthermore, toxicity study of the mixture after biodegradation of the pharmaceuticals showed that the solutions obtained after the process were approximately 65{\%} less toxic than the initial naproxen and diclofenac solutions.",
keywords = "Laccase, Immobilization, Electrospun materials, Environmental pollutants, Pharmaceuticals, Biodegradation",
author = "Jakub Zdarta and Katarzyna Jankowska and Marta Wyszowska and Ewa Kijeńska-Gawrońska and Agnieszka Zgoła-Grześkowiak and Manuel Pinelo and Meyer, {Anne S.} and Dariusz Moszyński and Teofil Jesionowski",
year = "2019",
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language = "English",
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journal = "Materials Science and Engineering C: Biomimetic Materials, Sensors and Systems",
issn = "0928-4931",
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Robust biodegradation of naproxen and diclofenac by laccase immobilized using electrospun nanofibers with enhanced stability and reusability. / Zdarta, Jakub; Jankowska, Katarzyna; Wyszowska, Marta; Kijeńska-Gawrońska, Ewa; Zgoła-Grześkowiak, Agnieszka; Pinelo, Manuel; Meyer, Anne S.; Moszyński, Dariusz; Jesionowski, Teofil.

In: Materials Science and Engineering C: Biomimetic Materials, Sensors and Systems, Vol. 103, 109789, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Robust biodegradation of naproxen and diclofenac by laccase immobilized using electrospun nanofibers with enhanced stability and reusability

AU - Zdarta, Jakub

AU - Jankowska, Katarzyna

AU - Wyszowska, Marta

AU - Kijeńska-Gawrońska, Ewa

AU - Zgoła-Grześkowiak, Agnieszka

AU - Pinelo, Manuel

AU - Meyer, Anne S.

AU - Moszyński, Dariusz

AU - Jesionowski, Teofil

PY - 2019

Y1 - 2019

N2 - Enzymatic biodegradation of pharmaceuticals, using enzymes such as laccase, is a green solution for the removal of toxic pollutants that has attracted growing interest over recent years. Moreover, the application of immobilized biocatalysts is relevant for industrial applications, due to the improved stability and reusability of the immobilized enzymes. Thus, in the present study, laccase was immobilized by adsorption and encapsulation using poly(l-lactic acid)-co-poly(ε-caprolactone) (PLCL) electrospun nanofibers as a tailor-made support. The produced biocatalytic systems were applied in the biodegradation of two commonly used anti-inflammatories, naproxen and diclofenac, which are present in wastewaters at environmentally relevant concentrations. The results showed that under optimal process conditions (temperature 25 °C, pH 5 and 3 for naproxen and diclofenac respectively), even from a solution at a concentration of 1 mg L−1, over 90% of both pharmaceuticals was removed by encapsulated laccase in batch mode. Both immobilized enzymes also exhibited high reusability: after five reaction cycles approximately 60% and 40% of naproxen and diclofenac were removed by encapsulated and adsorbed laccase respectively. In addition, a thorough analysis was made of the products of biodegradation of the two studied pollutants. Furthermore, toxicity study of the mixture after biodegradation of the pharmaceuticals showed that the solutions obtained after the process were approximately 65% less toxic than the initial naproxen and diclofenac solutions.

AB - Enzymatic biodegradation of pharmaceuticals, using enzymes such as laccase, is a green solution for the removal of toxic pollutants that has attracted growing interest over recent years. Moreover, the application of immobilized biocatalysts is relevant for industrial applications, due to the improved stability and reusability of the immobilized enzymes. Thus, in the present study, laccase was immobilized by adsorption and encapsulation using poly(l-lactic acid)-co-poly(ε-caprolactone) (PLCL) electrospun nanofibers as a tailor-made support. The produced biocatalytic systems were applied in the biodegradation of two commonly used anti-inflammatories, naproxen and diclofenac, which are present in wastewaters at environmentally relevant concentrations. The results showed that under optimal process conditions (temperature 25 °C, pH 5 and 3 for naproxen and diclofenac respectively), even from a solution at a concentration of 1 mg L−1, over 90% of both pharmaceuticals was removed by encapsulated laccase in batch mode. Both immobilized enzymes also exhibited high reusability: after five reaction cycles approximately 60% and 40% of naproxen and diclofenac were removed by encapsulated and adsorbed laccase respectively. In addition, a thorough analysis was made of the products of biodegradation of the two studied pollutants. Furthermore, toxicity study of the mixture after biodegradation of the pharmaceuticals showed that the solutions obtained after the process were approximately 65% less toxic than the initial naproxen and diclofenac solutions.

KW - Laccase

KW - Immobilization

KW - Electrospun materials

KW - Environmental pollutants

KW - Pharmaceuticals

KW - Biodegradation

U2 - 10.1016/j.msec.2019.109789

DO - 10.1016/j.msec.2019.109789

M3 - Journal article

VL - 103

JO - Materials Science and Engineering C: Biomimetic Materials, Sensors and Systems

JF - Materials Science and Engineering C: Biomimetic Materials, Sensors and Systems

SN - 0928-4931

M1 - 109789

ER -