RNF8 ubiquitylates histones at DNA double-strand breaks and promotes assembly of repair proteins

Niels Mailand, Simon Bekker-Jensen, Helene Faustrup Kildegaard, Fredrik Melander, Jiri Bartek, Claudia Lukas, Jiri Lukas

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Accumulation of repair proteins on damaged chromosomes is required to restore genomic integrity. However, the mechanisms of protein retention at the most destructive chromosomal lesions, the DNA double-strand breaks (DSBs), are poorly understood. We show that RNF8, a RING-finger ubiquitin ligase, rapidly assembles at DSBs via interaction of its FHA domain with the phosphorylated adaptor protein MDC1. This is accompanied by an increase in DSB-associated ubiquitylations and followed by accumulation of 53BP1 and BRCA1 repair proteins. Knockdown of RNF8 or disruption of its FHA or RING domains impaired DSB-associated ubiquitylation and inhibited retention of 53BP1 and BRCA1 at the DSB sites. In addition, we show that RNF8 can ubiquitylate histone H2A and H2AX, and that its depletion sensitizes cells to ionizing radiation. These data suggest that MDC1-mediated and RNF8-executed histone ubiquitylation protects genome integrity by licensing the DSB-flanking chromatin to concentrate repair factors near the DNA lesions.
Original languageEnglish
JournalCELL
Volume131
Issue number5
Pages (from-to)887-900
Number of pages14
ISSN0092-8674
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • DNA
  • Proteins
  • Signaling

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