RNA-Seq transcriptomics and pathway analyses reveal potential regulatory genes and molecular mechanisms in high- and low-residual feed intake in Nordic dairy cattle

M. S. Salleh, G. Mazzoni, J. K. Höglund, D. W. Olijhoek, P. Lund, P. Løvendahl, Haja Kadarmideen

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    Abstract

    The selective breeding of cattle with high-feed efficiencies (FE) is an important goal of beef and dairy cattle producers. Global gene expression patterns in relevant tissues can be used to study the functions of genes that are potentially involved in regulating FE. In the present study, high-throughput RNA sequencing data of liver biopsies from 19 dairy cows were used to identify differentially expressed genes (DEGs) between high- and low-FE groups of cows (based on Residual Feed Intake or RFI). Subsequently, a profile of the pathways connecting the DEGs to FE was generated, and a list of candidate genes and biomarkers was derived for their potential inclusion in breeding programmes to improve FE. The bovine RNA-Seq gene expression data from the liver was analysed to identify DEGs and, subsequently, identify the molecular mechanisms, pathways and possible candidate biomarkers of feed efficiency. On average, 57 million reads (short reads or short mRNA sequences 
    Original languageEnglish
    Article number258
    JournalB M C Genomics
    Volume18
    Issue number1
    Number of pages17
    ISSN1471-2164
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Dairy cattle
    • Differentially expressed genes
    • Feed efficiency
    • Pathways
    • RNA-Seq
    • Residual feed intake

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