Ribotyping and plasmid profiling of vibrio-anguillarum serovar o2 and vibrio-ordalii

T. Tiainen, Karl Pedersen, J.L. Larsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

One hundred and twenty-nine strains of Vibrio anguillarum serovar O2 and 14 strains of Vibrio ordalii were ribotyped and examined for plasmid contents. A total of 35 different ribotypes were detected. The V. anguillarum serovar O2 strains were divided into 32 different ribotypes. The V. ordalii strains showed three different ribotypes, clearly distinct from those of the V. anguillarum strains. Ribotypes were separated into seven clusters, of which one comprised the V. ordalii strains. Clustering of the strains indicated a genetic difference between North European and South European V. anguillarum O2 strains. Sere-subgroups O2a and O2b shared ribotypes; however, three of the clusters did not include O2a strains. All V. ordalii strains had a plasmid of 32 kb. This plasmid was not detected in any of the V. anguillarum strains. Seventeen different plasmid profiles with 17 different sized plasmids were detected among the V. anguillarum strains. Most of the plasmids were small (<6 kb) and found in several strains. Except for one South European strain, plasmids were detected only in the North European strains of V. anguillarum O2.
Original languageEnglish
JournalJournal of Applied Bacteriology
Volume79
Issue number4
Pages (from-to)384-392
Number of pages9
ISSN0021-8847
DOIs
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • Cluster Analysis
  • DNA, Bacterial
  • Plasmids
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • RNA, Ribosomal, 23S
  • Serotyping
  • Vibrio
  • MICROBIOLOGY
  • BIOTECHNOLOGY
  • RIBOSOMAL-RNA SEQUENCES
  • FISH
  • IDENTIFICATION
  • VIRULENCE
  • STRAINS
  • GENE
  • ANALYTICAL METHOD
  • CLUSTERS
  • FISH PATHOGEN
  • GENETICS
  • METHODS
  • SUBGROUPS
  • TAXONOMY
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio anguillarum Vibrio ordalli
  • Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] bacteria
  • Microorganisms (Microorganisms) - Microorganisms [01000] microorganism
  • Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] Pisces
  • 07516, Ecology: environmental biology - Wildlife management: aquatic
  • 10010, Comparative biochemistry
  • 10050, Biochemistry methods - General
  • 10052, Biochemistry methods - Nucleic acids, purines and pyrimidines
  • 10060, Biochemistry studies - General
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 10504, Biophysics - Methods and techniques
  • 10506, Biophysics - Molecular properties and macromolecules
  • 12502, Pathology - General
  • 30000, Bacteriology, general and systematic
  • 30500, Morphology and cytology of bacteria
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • 32000, Microbiological apparatus, methods and media
  • 34504, Immunology - Bacterial, viral and fungal
  • 36001, Medical and clinical microbiology - General and methods
  • 36002, Medical and clinical microbiology - Bacteriology
  • Chemical Coordination and Homeostasis
  • Conservation
  • Biochemistry and Molecular Biophysics
  • Cell Biology
  • Genetics
  • Immune System
  • Infection
  • Methods and Techniques
  • Pathology
  • Physiology
  • Systematics and Taxonomy
  • Wildlife Management

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