Rhodonellum psychrophilum gen. nov., sp nov., a novel psychrophilic and alkaliphilic bacterium of the phylum Bacteroidetes isolated from Greenland

Mariane Schmidt Thøgersen, Anders Prieme, Peter Stougaard

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A novel alkaliphilic and psychrophilic bacterium was isolated from the cold and alkaline ikaite tufa columns of the Ikka Fjord in south-west Greenland. According to 16S rRNA gene sequence analysis, strain GCM71T belonged to the family 'Flexibacteraceae' in the phylum Bacteroidetes. Strain GCM71T, together with five related isolates from ikaite columns, formed a separate cluster with 86-93% gene sequence similarity to their closest relative, Belliella baltica. The G+C content of the DNA from strain GCM71T was 43.1 mol%, whereas that of B. baltica was reported to be 35 mol%. DNA-DNA hybridization between strain GCM71T and B. baltica was 9.5%. The strain was red pigmented, Gram-negative, strictly aerobic with non-motile, rod-shaped cells. The optimal growth conditions for strain GCM71T were pH 9.2-10.0,5 degrees C and 0.6% NaCl. The fatty acid profile of the novel strain was dominated by branched and unsaturated fatty acids (90-97%), with a high abundance of iso-C17:1ω9c (17.5%), iso-C17:0 3-OH (17.5%) and summed feature 3, comprising iso-C15:0 2-OH and/or C16:1 ω7c (12.6%). Phylogenetic, chemotaxonomic and physiological characteristics showed that the novel strain could not be affiliated to any known genus. A new genus, Rhodonellum gen. nov., is proposed to accommodate the novel strain. Strain GCM71T (=DSM 17998T =LMG 23454T) is proposed as the type strain of the type species, Rhodonellum psychrophilum sp. nov.
Original languageEnglish
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume56
Issue numberPart 12
Pages (from-to)2887-2892
Number of pages6
ISSN1466-5026
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901*] Bacteroidetes higher_taxa Rhodonellum psychrophilum species new species, new genus description, strain-GCM71 Belliella baltica species
  • 00504, General biology - Taxonomy, nomenclature and terminology
  • 30000, Bacteriology, general and systematic
  • 30500, Morphology and cytology of bacteria
  • 31000, Physiology and biochemistry of bacteria
  • Systematics and Taxonomy

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