Rev-clip, a new approach to identify proteins interacting with rna

Sébastien Muller (Inventor), Christopher Workman (Inventor)

Research output: Patent

109 Downloads (Pure)

Abstract

The invention relates to methods for the global and specific isolation and characterization of the in vivo RNA-Binding Proteome (RBPome) present in a cell. The method is performed on cells that have been exposed to UV-light that serves to selectively cross-link cellular RNAs to their associated protein partners under physiological conditions. The method employs RNA splint-mediated ligation to RNA adapters, to selectively isolate cellular RNAs and their associated protein partners, and purification steps that ensure a higher yield and specificity of proteins considered as RBPs.
Original languageEnglish
IPCC12N15/10
Patent numberEP3296397
Filing date21/03/2018
Priority date20/09/2016
Priority numberEP20160189688
Publication statusPublished - 2018

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