Retinoic acid signalling in thymocytes regulates T cell development

Kerstin Wendland, Katarzyna Maria Sitnik, Knut Kotarsky, Andrea J. White, Graham Anderson, William Winston Agace

    Research output: Contribution to conferencePosterResearchpeer-review

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    Abstract

    The Vitamin A derivative retinoic acid (RA) works as a ligand for a family of nuclearRA receptors (RARα, RARβ and RARγ) which form heterodimers with retinoid Xreceptors (RXR). These complexes function as ligand-activated transcription factors,recognizing specific RA responsive elements in the regulatory regions of targetgenes. RA has been reported to play a direct role in regulating multiple aspects of peripheralT cell responses1, but whether endogenous RA signalling occurs in developingthymocytes and the potential impact of such signals in regulating T cell developmentremains unclear. To address this question, we have crossed dnRARα mice, that carry a dominantnegative(dn) RARα transgene behind a floxed transcriptional STOP cassette, withCD4Cre mice. In the resulting dnRARα-CD4Cre+ mice, CD4-promotor driven Creexpressionremoves the STOP cassette and leads to constitutive expression of thednRARα. This blocks RA signalling in developing thymocytes from the DN3/4 stageonwards and thus allows us to study the role of RA in T cell development
    Original languageEnglish
    Publication date2015
    Number of pages1
    Publication statusPublished - 2015
    Event1st International Venice Thymus Meeting - San Servolo Island, Italy
    Duration: 9 Apr 201513 Apr 2015

    Conference

    Conference1st International Venice Thymus Meeting
    CountryItaly
    CitySan Servolo Island
    Period09/04/201513/04/2015

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