Restriction Fragment Pattern (RFP) analysis of genomes from Danish isolates of Suid herpesvirus 1 (Aujeszky's disease virus)

Laurids Siig Christensen; K. J. Sørensen; J. C. Lei

doi:10.1007/BF01314422

Restriction Fragment Pattern (RFP) analysis of genomes from Danish isolates of Suid herpesvirus 1 (Aujeszky's disease virus)

Laurids Siig Christensen, K. J. Sørensen, J. C. Lei

National Food Institute

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Purified DNA from 42 isolates of Suid herpesvirus 1 (SHV-1) collected during 1985 from clinical outbreaks of Aujezsky's disease on Danish farms was compared by restriction fragment pattern (RFP) analysis. The BamHI generated RFPs were found to be distinguishable, thus confirming RFP analysis as a potential epidemiological tool. However, because of variations within labile genome regions of epidemiologically closely related isolates, it was concluded that comparisons of RFPs should be interpreted with great care and only on the basis of a comprehensive reference material. With this proviso, the changes in RFPs occurring during the spread of the infection might be useful markers in the evaluation of epidemiological relationships. A simple procedure for obtaining satisfactory amounts of purified SHV-1 DNA is described.

Original language	English
Journal	Archives of Virology
Volume	97
Issue number	3-4
Pages (from-to)	215-224
ISSN	0304-8608
DOIs	https://doi.org/10.1007/BF01314422
Publication status	Published - 1987

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10.1007/BF01314422

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Christensen, L. S., Sørensen, K. J., & Lei, J. C. (1987). Restriction Fragment Pattern (RFP) analysis of genomes from Danish isolates of Suid herpesvirus 1 (Aujeszky's disease virus). Archives of Virology, 97(3-4), 215-224. https://doi.org/10.1007/BF01314422

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abstract = "Purified DNA from 42 isolates of Suid herpesvirus 1 (SHV-1) collected during 1985 from clinical outbreaks of Aujezsky's disease on Danish farms was compared by restriction fragment pattern (RFP) analysis. The BamHI generated RFPs were found to be distinguishable, thus confirming RFP analysis as a potential epidemiological tool. However, because of variations within labile genome regions of epidemiologically closely related isolates, it was concluded that comparisons of RFPs should be interpreted with great care and only on the basis of a comprehensive reference material. With this proviso, the changes in RFPs occurring during the spread of the infection might be useful markers in the evaluation of epidemiological relationships. A simple procedure for obtaining satisfactory amounts of purified SHV-1 DNA is described.",

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year = "1987",

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T1 - Restriction Fragment Pattern (RFP) analysis of genomes from Danish isolates of Suid herpesvirus 1 (Aujeszky's disease virus)

AU - Christensen, Laurids Siig

AU - Sørensen, K. J.

AU - Lei, J. C.

PY - 1987

Y1 - 1987

N2 - Purified DNA from 42 isolates of Suid herpesvirus 1 (SHV-1) collected during 1985 from clinical outbreaks of Aujezsky's disease on Danish farms was compared by restriction fragment pattern (RFP) analysis. The BamHI generated RFPs were found to be distinguishable, thus confirming RFP analysis as a potential epidemiological tool. However, because of variations within labile genome regions of epidemiologically closely related isolates, it was concluded that comparisons of RFPs should be interpreted with great care and only on the basis of a comprehensive reference material. With this proviso, the changes in RFPs occurring during the spread of the infection might be useful markers in the evaluation of epidemiological relationships. A simple procedure for obtaining satisfactory amounts of purified SHV-1 DNA is described.

AB - Purified DNA from 42 isolates of Suid herpesvirus 1 (SHV-1) collected during 1985 from clinical outbreaks of Aujezsky's disease on Danish farms was compared by restriction fragment pattern (RFP) analysis. The BamHI generated RFPs were found to be distinguishable, thus confirming RFP analysis as a potential epidemiological tool. However, because of variations within labile genome regions of epidemiologically closely related isolates, it was concluded that comparisons of RFPs should be interpreted with great care and only on the basis of a comprehensive reference material. With this proviso, the changes in RFPs occurring during the spread of the infection might be useful markers in the evaluation of epidemiological relationships. A simple procedure for obtaining satisfactory amounts of purified SHV-1 DNA is described.

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DO - 10.1007/BF01314422

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JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 3-4

ER -