Restriction fragment length polymorphism of the pMJ101-like plasmid and ribotyping in the fish pathogen Vibrio ordalii

Karl Pedersen; S. Koblavi; T. Tiainen; P. A. D. Grimont

doi:10.1017/S0950268800001576

Restriction fragment length polymorphism of the pMJ101-like plasmid and ribotyping in the fish pathogen Vibrio ordalii

Karl Pedersen
, S. Koblavi
, T. Tiainen
, P. A. D. Grimont

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A total of 32 Vibrio ordalii strains were studied for their plasmid content and shown to carry a plasmid of approximately 32 kb. This plasmid was subsequently subjected to restriction fragment length polymorphism (RFLP) studies. Using Hind III, three different restriction patterns were identified while BamHI cleaved the plasmid into a single linear fragment. The results suggest that the 32 kb plasmid is highly conserved but that some variation in restriction pattern occurs. The same set of strains was subjected to ribotyping. Using Mill I, six different restriction patterns were demonstrated. Strains from the USA and Canada shared profiles with strains from Australia and Japan. Strains from Australia generated a single pattern whereas strains from North America were subdivided into three patterns, and the Japanese strains fell into five patterns. The results suggest that ribotyping in combination with RFLP studies of the pMJ101-like plasmid may be useful in epidemiological studies of V. ordalii.

Original language	English
Journal	Epidemiology and Infection
Volume	117
Issue number	2
Pages (from-to)	385-391
Number of pages	7
ISSN	0950-2688
DOIs	https://doi.org/10.1017/S0950268800001576
Publication status	Published - 1996
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Animals
Australia
DNA, Bacterial
Deoxyribonuclease HindIII
Fish Diseases
Japan
Molecular Epidemiology
North America
Plasmids
Polymorphism, Restriction Fragment Length
Serotyping
Vibrio
Vibrio Infections
EC 3.1.21.- Deoxyribonuclease HindIII
plasmid dna
type ii site specific deoxyribonuclease
article
australia
canada
fish
japan
nonhuman
plasmid
restriction fragment length polymorphism
ribotyping
united states
vibrio
Epidemiology, Molecular
INFECTIOUS
RIBOSOMAL-RNA
VIRULENCE PLASMID
ANGUILLARUM
PATTERNS
STRAINS
DIVERSITY
SALMON
DNA
Australasian region Australasian region
Australia Australasian region
Canada North America Nearctic region
Japan Asia Palearctic region
Nearctic region Nearctic region
North America Nearctic region
Palearctic region Palearctic region
USA North America Nearctic region
ANALYTICAL METHOD
BACTERIOLOGY
BAMH I GENE
EPIDEMIOLOGY
INFECTION
MLUI GENE
PATHOGEN
PMJ101-LIKE PLASMID
RESTRICTIONS FRAGMENT LENGTH POLYMORPHISM
RIBOTYPING
Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio ordalii
Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] fish Pisces
BAMH I 81295-09-2
31500, Genetics of bacteria and viruses
32000, Microbiological apparatus, methods and media
36002, Medical and clinical microbiology - Bacteriology
37400, Public health: microbiology - Public health microbiology
Genetics
Infection
Methods and Techniques

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10.1017/S0950268800001576

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title = "Restriction fragment length polymorphism of the pMJ101-like plasmid and ribotyping in the fish pathogen Vibrio ordalii",

abstract = "A total of 32 Vibrio ordalii strains were studied for their plasmid content and shown to carry a plasmid of approximately 32 kb. This plasmid was subsequently subjected to restriction fragment length polymorphism (RFLP) studies. Using Hind III, three different restriction patterns were identified while BamHI cleaved the plasmid into a single linear fragment. The results suggest that the 32 kb plasmid is highly conserved but that some variation in restriction pattern occurs. The same set of strains was subjected to ribotyping. Using Mill I, six different restriction patterns were demonstrated. Strains from the USA and Canada shared profiles with strains from Australia and Japan. Strains from Australia generated a single pattern whereas strains from North America were subdivided into three patterns, and the Japanese strains fell into five patterns. The results suggest that ribotyping in combination with RFLP studies of the pMJ101-like plasmid may be useful in epidemiological studies of V. ordalii.",

keywords = "Animals, Australia, DNA, Bacterial, Deoxyribonuclease HindIII, Fish Diseases, Japan, Molecular Epidemiology, North America, Plasmids, Polymorphism, Restriction Fragment Length, Serotyping, Vibrio, Vibrio Infections, EC 3.1.21.- Deoxyribonuclease HindIII, plasmid dna, type ii site specific deoxyribonuclease, article, australia, canada, fish, japan, nonhuman, plasmid, restriction fragment length polymorphism, ribotyping, united states, vibrio, Epidemiology, Molecular, INFECTIOUS, RIBOSOMAL-RNA, VIRULENCE PLASMID, ANGUILLARUM, PATTERNS, STRAINS, DIVERSITY, SALMON, DNA, Australasian region Australasian region, Australia Australasian region, Canada North America Nearctic region, Japan Asia Palearctic region, Nearctic region Nearctic region, North America Nearctic region, Palearctic region Palearctic region, USA North America Nearctic region, ANALYTICAL METHOD, BACTERIOLOGY, BAMH I GENE, EPIDEMIOLOGY, INFECTION, MLUI GENE, PATHOGEN, PMJ101-LIKE PLASMID, RESTRICTIONS FRAGMENT LENGTH POLYMORPHISM, RIBOTYPING, Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio ordalii, Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] fish Pisces, BAMH I 81295-09-2, 31500, Genetics of bacteria and viruses, 32000, Microbiological apparatus, methods and media, 36002, Medical and clinical microbiology - Bacteriology, 37400, Public health: microbiology - Public health microbiology, Genetics, Infection, Methods and Techniques",

author = "Karl Pedersen and S. Koblavi and T. Tiainen and Grimont, \{P. A. D.\}",

year = "1996",

doi = "10.1017/S0950268800001576",

language = "English",

volume = "117",

pages = "385--391",

journal = "Epidemiology and Infection",

issn = "0950-2688",

publisher = "Cambridge University Press",

number = "2",

}

TY - JOUR

T1 - Restriction fragment length polymorphism of the pMJ101-like plasmid and ribotyping in the fish pathogen Vibrio ordalii

AU - Pedersen, Karl

AU - Koblavi, S.

AU - Tiainen, T.

AU - Grimont, P. A. D.

PY - 1996

Y1 - 1996

N2 - A total of 32 Vibrio ordalii strains were studied for their plasmid content and shown to carry a plasmid of approximately 32 kb. This plasmid was subsequently subjected to restriction fragment length polymorphism (RFLP) studies. Using Hind III, three different restriction patterns were identified while BamHI cleaved the plasmid into a single linear fragment. The results suggest that the 32 kb plasmid is highly conserved but that some variation in restriction pattern occurs. The same set of strains was subjected to ribotyping. Using Mill I, six different restriction patterns were demonstrated. Strains from the USA and Canada shared profiles with strains from Australia and Japan. Strains from Australia generated a single pattern whereas strains from North America were subdivided into three patterns, and the Japanese strains fell into five patterns. The results suggest that ribotyping in combination with RFLP studies of the pMJ101-like plasmid may be useful in epidemiological studies of V. ordalii.

AB - A total of 32 Vibrio ordalii strains were studied for their plasmid content and shown to carry a plasmid of approximately 32 kb. This plasmid was subsequently subjected to restriction fragment length polymorphism (RFLP) studies. Using Hind III, three different restriction patterns were identified while BamHI cleaved the plasmid into a single linear fragment. The results suggest that the 32 kb plasmid is highly conserved but that some variation in restriction pattern occurs. The same set of strains was subjected to ribotyping. Using Mill I, six different restriction patterns were demonstrated. Strains from the USA and Canada shared profiles with strains from Australia and Japan. Strains from Australia generated a single pattern whereas strains from North America were subdivided into three patterns, and the Japanese strains fell into five patterns. The results suggest that ribotyping in combination with RFLP studies of the pMJ101-like plasmid may be useful in epidemiological studies of V. ordalii.

KW - Animals

KW - Australia

KW - DNA, Bacterial

KW - Deoxyribonuclease HindIII

KW - Fish Diseases

KW - Japan

KW - Molecular Epidemiology

KW - North America

KW - Plasmids

KW - Polymorphism, Restriction Fragment Length

KW - Serotyping

KW - Vibrio

KW - Vibrio Infections

KW - EC 3.1.21.- Deoxyribonuclease HindIII

KW - plasmid dna

KW - type ii site specific deoxyribonuclease

KW - article

KW - australia

KW - canada

KW - fish

KW - japan

KW - nonhuman

KW - plasmid

KW - restriction fragment length polymorphism

KW - ribotyping

KW - united states

KW - vibrio

KW - Epidemiology, Molecular

KW - INFECTIOUS

KW - RIBOSOMAL-RNA

KW - VIRULENCE PLASMID

KW - ANGUILLARUM

KW - PATTERNS

KW - STRAINS

KW - DIVERSITY

KW - SALMON

KW - DNA

KW - Australasian region Australasian region

KW - Australia Australasian region

KW - Canada North America Nearctic region

KW - Japan Asia Palearctic region

KW - Nearctic region Nearctic region

KW - North America Nearctic region

KW - Palearctic region Palearctic region

KW - USA North America Nearctic region

KW - ANALYTICAL METHOD

KW - BACTERIOLOGY

KW - BAMH I GENE

KW - EPIDEMIOLOGY

KW - INFECTION

KW - MLUI GENE

KW - PATHOGEN

KW - PMJ101-LIKE PLASMID

KW - RESTRICTIONS FRAGMENT LENGTH POLYMORPHISM

KW - RIBOTYPING

KW - Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Vibrio ordalii

KW - Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] fish Pisces

KW - BAMH I 81295-09-2

KW - 31500, Genetics of bacteria and viruses

KW - 32000, Microbiological apparatus, methods and media

KW - 36002, Medical and clinical microbiology - Bacteriology

KW - 37400, Public health: microbiology - Public health microbiology

KW - Genetics

KW - Infection

KW - Methods and Techniques

U2 - 10.1017/S0950268800001576

DO - 10.1017/S0950268800001576

M3 - Journal article

SN - 0950-2688

VL - 117

SP - 385

EP - 391

JO - Epidemiology and Infection

JF - Epidemiology and Infection

IS - 2

ER -

Restriction fragment length polymorphism of the pMJ101-like plasmid and ribotyping in the fish pathogen Vibrio ordalii

Abstract

UN SDGs

Keywords

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