Responses of barley root and shoot proteomes to long‐term nitrogen deficiency, short‐term nitrogen starvation and ammonium

Anders Laurell Blom Møller, Pai Pedas, Birgit Andersen, Birte Svensson, Jan K. Schoerring, Christine Finnie

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Cereals are major crops worldwide, and improvement of their nitrogen use efficiency is a crucial challenge. In this study proteins responding to N supply in barley roots and shoots were analysed using a proteomics approach, to provide insight into mechanisms of N uptake and assimilation. Control plants grown hydroponically for 33 d with 5 mm nitrate, plants grown under N deficiency (0.5 mm nitrate, 33 d) or short‐term N starvation (28 d with 5 mm nitrate followed by 5 d with no N source) were compared. N deficiency caused changes in C and N metabolism and ascorbate‐glutathione cycle enzymes in shoots and roots. N starvation altered proteins of amino acid metabolism in roots. Both treatments caused proteome changes in roots that could affect growth. Shoots of plants grown with ammonium as N source (28 d with 5 mm nitrate followed by 5 d with 5 mm ammonium) showed responses similar to N deficient shoots, characterized by turnover of ribulose 1·5‐bisphosphate carboxylase/oxygenase (Rubisco) and increases in proteins of the chloroplastic transcription and translation machinery. Identified proteins in 67 and 49 varying spots in roots and shoots respectively, corresponded to 62 functions and over 80 gene products, considerably advancing knowledge of N responses in barley.
    Original languageEnglish
    JournalPlant, Cell and Environment
    Volume34
    Issue number12
    Pages (from-to)2024-2037
    ISSN0140-7791
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Proteome analysis
    • Amino acid metabolism
    • Nitrate
    • Hydroponics
    • Redox regulation
    • Ascorbate-glutathione cycle
    • Mass spectrometry
    • Twodimensional gel electrophoresis
    • Carbon metabolism

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