Abstract
The method of indolo-.alpha.-pyrone fluorescence-determination of IAA was investigated to study possible interference from 4-chloro-indoleacetic acid and 5-hydroxyindoleacetic acid, which occur naturally. Both compounds show about 40% of the fluorescence of IAA after conversion into their .alpha.-pyrones. Other halogenated indoleacetic acids show between zero and 60% of the fluorescence of IAA. Apparently the concentration of IAA cannot be determined in crude extracts in the presence of 4-chloro- or 5-hydroxy-indoleacetic acid, because separate determinations of each of these compounds are not possible by changing the excitation or fluorescence wave-lengths of the testing equipment.
| Original language | English |
|---|---|
| Journal | Physiologia Plantarum |
| Volume | 43 |
| Issue number | 1 |
| Pages (from-to) | 62-64 |
| ISSN | 0031-9317 |
| Publication status | Published - 1978 |