Response of Substituted Indoleacetic Acids in the Indolo-alpha-pyrone Fluorescence Determination

Kjeld Christensen Engvild, M. Böttger, P. Kaiser

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The method of indolo-.alpha.-pyrone fluorescence-determination of IAA was investigated to study possible interference from 4-chloro-indoleacetic acid and 5-hydroxyindoleacetic acid, which occur naturally. Both compounds show about 40% of the fluorescence of IAA after conversion into their .alpha.-pyrones. Other halogenated indoleacetic acids show between zero and 60% of the fluorescence of IAA. Apparently the concentration of IAA cannot be determined in crude extracts in the presence of 4-chloro- or 5-hydroxy-indoleacetic acid, because separate determinations of each of these compounds are not possible by changing the excitation or fluorescence wave-lengths of the testing equipment.
    Original languageEnglish
    JournalPhysiologia Plantarum
    Volume43
    Issue number1
    Pages (from-to)62-64
    ISSN0031-9317
    Publication statusPublished - 1978

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