Response of porcine peripheral blood mononuclear cells to CpG-containing oligodeoxynucleotide

Søren Kamstrup, D. Verthelyi, D.M. Klinman

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Exposure to bacterial DNA generates a "danger signal" that stimulates cellular elements of the mammalian immune system to proliferate and/or secrete cytokines. Stimulation is critically dependent on hexameric motifs that contain an unmethylated CpG dinucleotide: these are commonly found in bacterial but not vertebrate DNA. Different motifs are optimally stimulatory in different species. This work examines whether oligodeoxynucleotides (ODNs) containing CpG motifs stimulate peripheral blood mononuclear cells from pigs. Results show that pigs respond to CpG ODN by proliferating and secreting IL-6, IL-12 and TNF-alpha. By screening a large panel (>100) of ODNs, the palindromic hexamer 'ATCGAT' was identified as being optimally active in all animals examined (N = 10). These findings are the first to establish the immunostimulatory activity of CpG ODN in pigs, and suggest that the therapeutic uses envisioned for these ODNs (as vaccine adjuvants and immunoprotective agents) may be applicable to husbandry animals.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume78
    Issue number4
    Pages (from-to)353-362
    ISSN0378-1135
    Publication statusPublished - 2001

    Keywords

    • PBMC
    • oligodeoxynucleotides
    • pigs
    • CpG
    • immunostimulation

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