Response Factors Enable Rapid Quantitative 2D NMR Analysis in Catalytic Biomass Conversion to Renewable Chemicals

Samuel Gilbert Elliot*, Irene Tosi, Anders Riisager, Esben Taarning, Sebastian Meier

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Carbohydrate conversion offers access to a variety of chemicals with diverse functionalities. An accurate analysis of the multiple products in post-reaction material is indispensable for enabling good atom economy in biorefining. A certain need for reconsidering current analytical approaches to chemocatalytic biomass conversion is witnessed by the often poor carbon balances that are reported for carbohydrate conversion processes. Carbohydrate conversion usually includes isomerization and/or dehydration, therefore analytical approaches that are suitable for the distinction and concurrent quantification of isomers are desirable for developing sustainable processes towards known and new chemicals. Quantitative 1D NMR spectroscopy can be used to determine absolute concentrations in the absence of purified reference compounds and can thereafter be used to obtain response factors in other analytical methods resolving the compounds of interest. Here, we show that this approach is applicable for obtaining response factors relative to an internal standard for rapid, highly resolved 2D NMR spectra at natural isotopic abundance. Following calibration, this approach provides a limit of quantification in the order of 0.8 mM within an experiment time of a few minutes. The approach is particularly beneficial for the quantification of compounds at low concentrations, for instance in initial rate experiments, and for the quantification of low populated reaction intermediates.
Original languageEnglish
JournalTopics in Catalysis
Volume62
Issue number7-11
Pages (from-to)580-598
Number of pages9
ISSN1022-5528
DOIs
Publication statusPublished - 2019

Keywords

  • Biomass
  • Catalysis
  • qNMR
  • Quantitative analysis
  • Reference standard
  • Response factor

Cite this

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title = "Response Factors Enable Rapid Quantitative 2D NMR Analysis in Catalytic Biomass Conversion to Renewable Chemicals",
abstract = "Carbohydrate conversion offers access to a variety of chemicals with diverse functionalities. An accurate analysis of the multiple products in post-reaction material is indispensable for enabling good atom economy in biorefining. A certain need for reconsidering current analytical approaches to chemocatalytic biomass conversion is witnessed by the often poor carbon balances that are reported for carbohydrate conversion processes. Carbohydrate conversion usually includes isomerization and/or dehydration, therefore analytical approaches that are suitable for the distinction and concurrent quantification of isomers are desirable for developing sustainable processes towards known and new chemicals. Quantitative 1D NMR spectroscopy can be used to determine absolute concentrations in the absence of purified reference compounds and can thereafter be used to obtain response factors in other analytical methods resolving the compounds of interest. Here, we show that this approach is applicable for obtaining response factors relative to an internal standard for rapid, highly resolved 2D NMR spectra at natural isotopic abundance. Following calibration, this approach provides a limit of quantification in the order of 0.8 mM within an experiment time of a few minutes. The approach is particularly beneficial for the quantification of compounds at low concentrations, for instance in initial rate experiments, and for the quantification of low populated reaction intermediates.",
keywords = "Biomass, Catalysis, qNMR, Quantitative analysis, Reference standard, Response factor",
author = "Elliot, {Samuel Gilbert} and Irene Tosi and Anders Riisager and Esben Taarning and Sebastian Meier",
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language = "English",
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publisher = "Springer New York",
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Response Factors Enable Rapid Quantitative 2D NMR Analysis in Catalytic Biomass Conversion to Renewable Chemicals. / Elliot, Samuel Gilbert; Tosi, Irene; Riisager, Anders; Taarning, Esben; Meier, Sebastian.

In: Topics in Catalysis, Vol. 62, No. 7-11, 2019, p. 580-598.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Response Factors Enable Rapid Quantitative 2D NMR Analysis in Catalytic Biomass Conversion to Renewable Chemicals

AU - Elliot, Samuel Gilbert

AU - Tosi, Irene

AU - Riisager, Anders

AU - Taarning, Esben

AU - Meier, Sebastian

PY - 2019

Y1 - 2019

N2 - Carbohydrate conversion offers access to a variety of chemicals with diverse functionalities. An accurate analysis of the multiple products in post-reaction material is indispensable for enabling good atom economy in biorefining. A certain need for reconsidering current analytical approaches to chemocatalytic biomass conversion is witnessed by the often poor carbon balances that are reported for carbohydrate conversion processes. Carbohydrate conversion usually includes isomerization and/or dehydration, therefore analytical approaches that are suitable for the distinction and concurrent quantification of isomers are desirable for developing sustainable processes towards known and new chemicals. Quantitative 1D NMR spectroscopy can be used to determine absolute concentrations in the absence of purified reference compounds and can thereafter be used to obtain response factors in other analytical methods resolving the compounds of interest. Here, we show that this approach is applicable for obtaining response factors relative to an internal standard for rapid, highly resolved 2D NMR spectra at natural isotopic abundance. Following calibration, this approach provides a limit of quantification in the order of 0.8 mM within an experiment time of a few minutes. The approach is particularly beneficial for the quantification of compounds at low concentrations, for instance in initial rate experiments, and for the quantification of low populated reaction intermediates.

AB - Carbohydrate conversion offers access to a variety of chemicals with diverse functionalities. An accurate analysis of the multiple products in post-reaction material is indispensable for enabling good atom economy in biorefining. A certain need for reconsidering current analytical approaches to chemocatalytic biomass conversion is witnessed by the often poor carbon balances that are reported for carbohydrate conversion processes. Carbohydrate conversion usually includes isomerization and/or dehydration, therefore analytical approaches that are suitable for the distinction and concurrent quantification of isomers are desirable for developing sustainable processes towards known and new chemicals. Quantitative 1D NMR spectroscopy can be used to determine absolute concentrations in the absence of purified reference compounds and can thereafter be used to obtain response factors in other analytical methods resolving the compounds of interest. Here, we show that this approach is applicable for obtaining response factors relative to an internal standard for rapid, highly resolved 2D NMR spectra at natural isotopic abundance. Following calibration, this approach provides a limit of quantification in the order of 0.8 mM within an experiment time of a few minutes. The approach is particularly beneficial for the quantification of compounds at low concentrations, for instance in initial rate experiments, and for the quantification of low populated reaction intermediates.

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KW - Catalysis

KW - qNMR

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KW - Reference standard

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