Rescue of the highly virulent classical swine fever virus strain “Koslov” from cloned cDNA and first insights into genome variations relevant for virulence

Ulrik Fahnøe, Anders Gorm Pedersen, Peter Christian Risager, Jens Nielsen, Graham Belsham, Dirk Höper, Martin Beer, Thomas Bruun Rasmussen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Classical swine fever virus (CSFV) strain “Koslov” is highly virulent with a mortality rate of up to 100% in pigs. In this study, we modified non-functional cDNAs generated from the blood of Koslov virus infected pigs bysite-directed mutagenesis, removing non-synonymous mutations step-by-step, thereby producing genomes encoding the consensus amino acid sequence. Viruses rescued from the construct corresponding to the inferred parental form were highly virulent, when tested in pigs, with infected animals displaying pronounced clinical symptoms leading to high mortality. The reconstruction therefore gave rise to a functional cDNA corresponding to the highly virulent Koslov strain of CSFV. It could be demonstrated that two single amino acid changes (S763L and P968H) in the surface structural protein E2 resulted in attenuation in the porcine infection system while another single amino acid change within the nonstructural protein NS3 (D2183G) reduced virus growth within cells in vitro.
    Original languageEnglish
    JournalVirology
    Volume468-470
    Pages (from-to)379-387
    Number of pages9
    ISSN0042-6822
    DOIs
    Publication statusPublished - 2014

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