Reprogramming amino acid catabolism in CHO cells with CRISPR-Cas9 genome editing improves cell growth and reduces by-product secretion

Daniel Ley, Sara Pereira, Lasse Ebdrup Pedersen, Johnny Arnsdorf, Hooman Hefzi, Anne Mathilde Lund, Tae Kwang Ha, Tune Wulff, Helene Faustrup Kildegaard, Mikael Rørdam Andersen

Research output: Contribution to conferencePosterResearchpeer-review

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Abstract

CHO cells primarily utilize amino acids for three processes: biomass synthesis, recombinant protein production and catabolism. In this work, we disrupted 9 amino acid catabolic genes participating in 7 dierent catabolic pathways, to increase synthesis of biomass and recombinant protein, while reducing production of growth-inhibiting metabolic by-products from amino acid catabolism.
Original languageEnglish
Publication date2017
Number of pages1
Publication statusPublished - 2017

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