TY - CONF
T1 - Reprogramming amino acid catabolism in CHO cells with CRISPR-Cas9 genome editing improves cell growth and reduces by-product secretion
AU - Ley, Daniel
AU - Pereira, Sara
AU - Pedersen, Lasse Ebdrup
AU - Arnsdorf, Johnny
AU - Hefzi, Hooman
AU - Lund, Anne Mathilde
AU - Kwang Ha, Tae
AU - Wulff, Tune
AU - Kildegaard, Helene Faustrup
AU - Andersen, Mikael Rørdam
PY - 2017
Y1 - 2017
N2 - CHO cells primarily utilize amino acids for three processes: biomass synthesis, recombinant protein production and catabolism. In this work, we disrupted 9 amino acid catabolic genes participating in 7 dierent catabolic pathways, to increase synthesis of biomass and recombinant protein, while reducing production of growth-inhibiting metabolic by-products from amino acid catabolism.
AB - CHO cells primarily utilize amino acids for three processes: biomass synthesis, recombinant protein production and catabolism. In this work, we disrupted 9 amino acid catabolic genes participating in 7 dierent catabolic pathways, to increase synthesis of biomass and recombinant protein, while reducing production of growth-inhibiting metabolic by-products from amino acid catabolism.
M3 - Poster
ER -