TY - JOUR
T1 - Reproductive and behavioral effects of diisononyl phthalate (DINP) in perinatally exposed rats
AU - Boberg, Julie
AU - Christiansen, Sofie
AU - Petersen, Marta Axelstad
AU - Kledal, Thuri Seidler
AU - Vinggaard, Anne Marie
AU - Dalgaard, Majken
AU - Nellemann, Christine Lydia
AU - Hass, Ulla
PY - 2011
Y1 - 2011
N2 - Diisononyl phthalate (DINP) is a plasticizer abundantly used in consumer products as a substitute for other plasticizers prohibited in certain products due to reproductive toxicity. As anti-androgenic effects of DINP are suspected, DINP effects on reproduction and sexually dimorphic behavior were studied.Pregnant Wistar rats were gavaged from gestation day 7 to postnatal day (PND) 17 with vehicle, 300, 600, 750 or 900mg DINP/kg bw/day.In fetal testes histopathological effects typical of phthalates were observed. In male offspring, DINP caused increased nipple retention, reduced anogenital distance, reduced sperm motility and increased sperm count. DINP affected spatial learning as female offspring performed better than controls and similarly to control males in the Morris Water Maze, indicating masculinization of behavior in DINP exposed females.These results show that DINP causes anti-androgenic effects on reproductive development, though less potent than DEHP, DBP and BBP, and further safety evaluation of DINP appears warranted.
AB - Diisononyl phthalate (DINP) is a plasticizer abundantly used in consumer products as a substitute for other plasticizers prohibited in certain products due to reproductive toxicity. As anti-androgenic effects of DINP are suspected, DINP effects on reproduction and sexually dimorphic behavior were studied.Pregnant Wistar rats were gavaged from gestation day 7 to postnatal day (PND) 17 with vehicle, 300, 600, 750 or 900mg DINP/kg bw/day.In fetal testes histopathological effects typical of phthalates were observed. In male offspring, DINP caused increased nipple retention, reduced anogenital distance, reduced sperm motility and increased sperm count. DINP affected spatial learning as female offspring performed better than controls and similarly to control males in the Morris Water Maze, indicating masculinization of behavior in DINP exposed females.These results show that DINP causes anti-androgenic effects on reproductive development, though less potent than DEHP, DBP and BBP, and further safety evaluation of DINP appears warranted.
KW - Rat
KW - Behavior
KW - Semen quality
KW - Phthalate
KW - Toxicity
KW - Anti-androgenic
U2 - 10.1016/j.reprotox.2010.11.001
DO - 10.1016/j.reprotox.2010.11.001
M3 - Journal article
SN - 0890-6238
VL - 31
SP - 200
EP - 209
JO - Reproductive Toxicology
JF - Reproductive Toxicology
IS - 2
ER -