Replication and clearance of respiratory syncytial virus - Apoptosis is an important pathway of virus clearance after experimental infection with bovine respiratory syncytial virus

B. Viuff, Kirsten Tjørnehøj, Lars Erik Larsen, C.M. Røntved, Åse Uttenthal, L. Rønsholt, Søren Alexandersen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Human respiratory syncytial virus is an important cause of severe respiratory disease in young children, the elderly, and in immunocompromised adults. Similarly, bovine respiratory syncytial virus (BRSV) is causing severe, sometimes fatal, respiratory disease in calves. Both viruses are pneumovirus and the infections with human respiratory syncytial. virus and BRSV have similar clinical, pathological, and epidemiological characteristics. In this study we used experimental BRSV infection in calves as a model of respiratory syncytial virus infection to demonstrate important aspects of viral replication and clearance in a natural target animal. Replication of BRSV was demonstrated in the luminal part of the respiratory epithelial cells and replication in the upper respiratory tract preceded the replication in the lower respiratory tract. Virus excreted to the lumen of the respiratory tract was cleared by neutrophils whereas apoptosis was an important way of clearance of BRSV-infected epithelial cells. Neighboring cells, which probably were epithelial cells, phagocytized the BRSV-infected apoptotic cells. The number of both CD4+ and CD8+ T cells increased during the course of infection, but the T cells were not found between the epithelial cells of the bronchi up until apoptosis was no longer detected, thus in the bronchi there was no indication of direct contact-dependent T-cell-mediated cytotoxicity in the primary infection.
    Original languageEnglish
    JournalAmerican Journal of Pathology
    Volume161
    Issue number6
    Pages (from-to)2195-2207
    ISSN0002-9440
    Publication statusPublished - 2002

    Cite this

    Viuff, B., Tjørnehøj, K., Larsen, L. E., Røntved, C. M., Uttenthal, Å., Rønsholt, L., & Alexandersen, S. (2002). Replication and clearance of respiratory syncytial virus - Apoptosis is an important pathway of virus clearance after experimental infection with bovine respiratory syncytial virus. American Journal of Pathology, 161(6), 2195-2207.
    Viuff, B. ; Tjørnehøj, Kirsten ; Larsen, Lars Erik ; Røntved, C.M. ; Uttenthal, Åse ; Rønsholt, L. ; Alexandersen, Søren. / Replication and clearance of respiratory syncytial virus - Apoptosis is an important pathway of virus clearance after experimental infection with bovine respiratory syncytial virus. In: American Journal of Pathology. 2002 ; Vol. 161, No. 6. pp. 2195-2207.
    @article{74ad0332e09c4ce2aa4603801185a527,
    title = "Replication and clearance of respiratory syncytial virus - Apoptosis is an important pathway of virus clearance after experimental infection with bovine respiratory syncytial virus",
    abstract = "Human respiratory syncytial virus is an important cause of severe respiratory disease in young children, the elderly, and in immunocompromised adults. Similarly, bovine respiratory syncytial virus (BRSV) is causing severe, sometimes fatal, respiratory disease in calves. Both viruses are pneumovirus and the infections with human respiratory syncytial. virus and BRSV have similar clinical, pathological, and epidemiological characteristics. In this study we used experimental BRSV infection in calves as a model of respiratory syncytial virus infection to demonstrate important aspects of viral replication and clearance in a natural target animal. Replication of BRSV was demonstrated in the luminal part of the respiratory epithelial cells and replication in the upper respiratory tract preceded the replication in the lower respiratory tract. Virus excreted to the lumen of the respiratory tract was cleared by neutrophils whereas apoptosis was an important way of clearance of BRSV-infected epithelial cells. Neighboring cells, which probably were epithelial cells, phagocytized the BRSV-infected apoptotic cells. The number of both CD4+ and CD8+ T cells increased during the course of infection, but the T cells were not found between the epithelial cells of the bronchi up until apoptosis was no longer detected, thus in the bronchi there was no indication of direct contact-dependent T-cell-mediated cytotoxicity in the primary infection.",
    author = "B. Viuff and Kirsten Tj{\o}rneh{\o}j and Larsen, {Lars Erik} and C.M. R{\o}ntved and {\AA}se Uttenthal and L. R{\o}nsholt and S{\o}ren Alexandersen",
    year = "2002",
    language = "English",
    volume = "161",
    pages = "2195--2207",
    journal = "American Journal of Pathology",
    issn = "0002-9440",
    publisher = "Elsevier",
    number = "6",

    }

    Viuff, B, Tjørnehøj, K, Larsen, LE, Røntved, CM, Uttenthal, Å, Rønsholt, L & Alexandersen, S 2002, 'Replication and clearance of respiratory syncytial virus - Apoptosis is an important pathway of virus clearance after experimental infection with bovine respiratory syncytial virus', American Journal of Pathology, vol. 161, no. 6, pp. 2195-2207.

    Replication and clearance of respiratory syncytial virus - Apoptosis is an important pathway of virus clearance after experimental infection with bovine respiratory syncytial virus. / Viuff, B.; Tjørnehøj, Kirsten; Larsen, Lars Erik; Røntved, C.M.; Uttenthal, Åse; Rønsholt, L.; Alexandersen, Søren.

    In: American Journal of Pathology, Vol. 161, No. 6, 2002, p. 2195-2207.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Replication and clearance of respiratory syncytial virus - Apoptosis is an important pathway of virus clearance after experimental infection with bovine respiratory syncytial virus

    AU - Viuff, B.

    AU - Tjørnehøj, Kirsten

    AU - Larsen, Lars Erik

    AU - Røntved, C.M.

    AU - Uttenthal, Åse

    AU - Rønsholt, L.

    AU - Alexandersen, Søren

    PY - 2002

    Y1 - 2002

    N2 - Human respiratory syncytial virus is an important cause of severe respiratory disease in young children, the elderly, and in immunocompromised adults. Similarly, bovine respiratory syncytial virus (BRSV) is causing severe, sometimes fatal, respiratory disease in calves. Both viruses are pneumovirus and the infections with human respiratory syncytial. virus and BRSV have similar clinical, pathological, and epidemiological characteristics. In this study we used experimental BRSV infection in calves as a model of respiratory syncytial virus infection to demonstrate important aspects of viral replication and clearance in a natural target animal. Replication of BRSV was demonstrated in the luminal part of the respiratory epithelial cells and replication in the upper respiratory tract preceded the replication in the lower respiratory tract. Virus excreted to the lumen of the respiratory tract was cleared by neutrophils whereas apoptosis was an important way of clearance of BRSV-infected epithelial cells. Neighboring cells, which probably were epithelial cells, phagocytized the BRSV-infected apoptotic cells. The number of both CD4+ and CD8+ T cells increased during the course of infection, but the T cells were not found between the epithelial cells of the bronchi up until apoptosis was no longer detected, thus in the bronchi there was no indication of direct contact-dependent T-cell-mediated cytotoxicity in the primary infection.

    AB - Human respiratory syncytial virus is an important cause of severe respiratory disease in young children, the elderly, and in immunocompromised adults. Similarly, bovine respiratory syncytial virus (BRSV) is causing severe, sometimes fatal, respiratory disease in calves. Both viruses are pneumovirus and the infections with human respiratory syncytial. virus and BRSV have similar clinical, pathological, and epidemiological characteristics. In this study we used experimental BRSV infection in calves as a model of respiratory syncytial virus infection to demonstrate important aspects of viral replication and clearance in a natural target animal. Replication of BRSV was demonstrated in the luminal part of the respiratory epithelial cells and replication in the upper respiratory tract preceded the replication in the lower respiratory tract. Virus excreted to the lumen of the respiratory tract was cleared by neutrophils whereas apoptosis was an important way of clearance of BRSV-infected epithelial cells. Neighboring cells, which probably were epithelial cells, phagocytized the BRSV-infected apoptotic cells. The number of both CD4+ and CD8+ T cells increased during the course of infection, but the T cells were not found between the epithelial cells of the bronchi up until apoptosis was no longer detected, thus in the bronchi there was no indication of direct contact-dependent T-cell-mediated cytotoxicity in the primary infection.

    M3 - Journal article

    VL - 161

    SP - 2195

    EP - 2207

    JO - American Journal of Pathology

    JF - American Journal of Pathology

    SN - 0002-9440

    IS - 6

    ER -