Abstract
Enzymatic conversion of pharmaceutically active ingredients (API), using
immobilized enzymes should be considered as a promising industrial tool
due to improved reusability and stability of the biocatalysts at harsh
process conditions. Therefore, in this study horseradish peroxidase was
immobilized into sodium alginate capsules and then trapped into
poly(vinyl chloride) electrospun fibers to provide additional enzyme
stabilization and protection against the negative effect of harsh
process conditions. Due to encapsulation immobilization, 100% of
immobilization yield was achieved leading to loading of 25 μg of enzyme
in 1 mg of the support. Immobilized in such a way, enzyme showed over
80% activity retention. Further, only slight changes in kinetic
parameters of free (Km = 1.54 mM) and immobilized horseradish peroxidase (Km
= 1.83 mM) were noticed, indicating retention of high catalytic
properties and high substrate affinity by encapsulated biocatalyst.
Encapsulated horseradish peroxidase was tested in biodegradation of two
frequently occurring in wastewater API, sulfamethoxazole (antibiotic)
and carbamazepine (anticonvulsant). Over 80% of both pharmaceutics was
removed by immobilized enzyme after 24 h of the process from the
solution at a concentration of 1 mg/L, under optimal conditions, which
were found to be pH 7, temperature 25 °C and 2 mM of H2O2.
However, even from 10 mg/L solutions, it was possible to remove over
40% of both pharmaceuticals. Finally, the reusability and storage
stability study of immobilized horseradish peroxidase showed retention
of over 60% of initial activity after 20 days of storage at 4 °C and
after 10 repeated catalytic cycles, indicating great practical
application potential. By contrast, the free enzyme showed less than 20%
of its initial activity after 20 days of storage and exhibited no
recycling potential.
Original language | English |
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Article number | 272 |
Journal | International Journal of Molecular Sciences |
Volume | 23 |
Issue number | 1 |
Number of pages | 16 |
ISSN | 1661-6596 |
DOIs | |
Publication status | Published - 2022 |
Keywords
- Biodegradation
- Electrospinning
- Encapsulation
- Enzyme immobilization
- Horseradish peroxidase
- Pharmaceuticals