Regulation of microRNAs miR-30a and miR-143 in cerebral vasculature after experimental subarachnoid hemorrhage in rats

Anne Holt Muller, Gro Klitgaard Povlsen, Claus Heiner Bang-Berthelsen, Lars Schack Kruse, Janne Nielsen, Karin Warfvinge, Lars Edvinsson

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Background: microRNAs (miRNAs) are important regulators of translation and have been implicated in the pathogenesis of a number of cardiovascular diseases, including stroke, and suggested as possible prognostic biomarkers. Our aim was to identify miRNAs that are differentially regulated in cerebral arteries after subarachnoid hemorrhage (SAH), using a rat injection model of SAH and a qPCR-based screen of 728 rat miRNAs. Additionally, serum was analyzed for a possible spill-over to the circulation of regulated miRNAs from the vessel walls.Results: We identified 482 different miRNAs expressed in cerebral arteries post-SAH. Two miRNAs, miR-30a and miR-143, were significantly upregulated in cerebral arteries after SAH when compared to sham-operated animals. However, none of these exhibited significantly altered serum levels after SAH versus post-sham surgery. The most robust upregulation was seen for miR-143, which has several predicted targets and is a strong regulator of vascular morphology. We hypothesize that miR-30a and miR-143 may play a role in the vascular wall changes seen after SAHConclusions: We report that miR-30a and miR-143 in the cerebral arteries show significant changes over time after SAH, but do not differ from sham-operated rats at 24 h post-SAH. Although this finding suggests interesting novel possible mechanisms involved in post-SAH cerebrovascular changes, the lack of regulation of these miRNAs in serum excludes their use as blood-borne biomarkers for cerebrovascular changes following SAH.
Original languageEnglish
Article number119
JournalB M C Genomics
Issue number1
Number of pages8
Publication statusPublished - 2015
Externally publishedYes


  • Biotechnology
  • Genetics
  • Medicine (all)
  • Animal model
  • Artery
  • Biomarker
  • Non-coding RNA
  • SAH
  • microRNA
  • microRNA 143
  • microRNA 30a
  • unclassified drug
  • MIRN143 microRNA, rat
  • MIRN30 microRNA, rat
  • animal experiment
  • animal model
  • animal tissue
  • Article
  • blood level
  • blood vessel wall
  • brain artery
  • controlled study
  • gene expression
  • male
  • nonhuman
  • rat
  • subarachnoid hemorrhage
  • upregulation
  • animal
  • blood
  • disease model
  • gene expression regulation
  • genetics
  • human
  • metabolism
  • pathology
  • surgery
  • Animalia
  • Rattus
  • Animals
  • Cerebral Arteries
  • Disease Models, Animal
  • Gene Expression Regulation
  • Humans
  • MicroRNAs
  • Rats
  • Subarachnoid Hemorrhage
  • Genetics - General
  • Genetics - Animal
  • Cardiovascular system - Physiology and biochemistry
  • Cardiovascular system - Blood vessel pathology
  • Blood - Blood and lymph studies
  • Blood - Blood cell studies
  • Muscle - Physiology and biochemistry
  • Nervous system - Physiology and biochemistry
  • Nervous system - Pathology
  • Animals, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Rodents, Vertebrates
  • angiogenesis
  • cerebrovascular change
  • vascular morphology


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