Refining the LPS-Antigen in Salmonella Antibody Elisa for Poultry Enhanced Specificity without Impairing Sensitivity

Klara Tølbøl Lauritsen, Peter Lind, Joan Klausen, Lars Ole Andresen

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    Abstract

    In the Danish serological surveillance for Salmonella in poultry (serum and egg yolk) a mix-ELISA is used, based on S. typhimurium and S. enteritidis antigens (Feld et al., 2000). When we evaluated results of the test retrospectively, over the years an unacceptably large fraction of seropositive findings could not be confirmed by the subsequent confirmatory bacteriological sampling in the herd. Therefore we tried to enhance specificity of the ELISA, without losing sensitivity, by refining the antigens used.
    Original languageEnglish
    Title of host publicationAbstract Book and Final Programme - 3rd European EAVLD Congress
    Number of pages1
    Place of PublicationPisa
    Publication date2014
    Article number143
    Publication statusPublished - 2014
    Event3rd European Association of Veterinary Laboratory Diagnosticians - Pisa, Italy
    Duration: 12 Oct 201415 Oct 2014

    Conference

    Conference3rd European Association of Veterinary Laboratory Diagnosticians
    CountryItaly
    CityPisa
    Period12/10/201415/10/2014

    Keywords

    • Salmonella
    • ELISA
    • Poultry

    Cite this

    Lauritsen, K. T., Lind, P., Klausen, J., & Andresen, L. O. (2014). Refining the LPS-Antigen in Salmonella Antibody Elisa for Poultry Enhanced Specificity without Impairing Sensitivity. In Abstract Book and Final Programme - 3rd European EAVLD Congress [143].