Refining the LPS-Antigen in Salmonella Antibody Elisa for Poultry Enhanced Specificity without Impairing Sensitivity

Klara Tølbøl Lauritsen; Peter Lind; Joan Klausen; Lars Ole Andresen

Refining the LPS-Antigen in Salmonella Antibody Elisa for Poultry Enhanced Specificity without Impairing Sensitivity

Klara Tølbøl Lauritsen, Peter Lind, Joan Klausen, Lars Ole Andresen

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research › peer-review

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Abstract

In the Danish serological surveillance for Salmonella in poultry (serum and egg yolk) a mix-ELISA is used, based on S. typhimurium and S. enteritidis antigens (Feld et al., 2000). When we evaluated results of the test retrospectively, over the years an unacceptably large fraction of seropositive findings could not be confirmed by the subsequent confirmatory bacteriological sampling in the herd. Therefore we tried to enhance specificity of the ELISA, without losing sensitivity, by refining the antigens used.

Original language	English
Title of host publication	Abstract Book and Final Programme - 3rd European EAVLD Congress
Number of pages	1
Place of Publication	Pisa
Publication date	2014
Article number	143
Publication status	Published - 2014
Event	3rd European Association of Veterinary Laboratory Diagnosticians - Pisa, Italy Duration: 12 Oct 2014 → 15 Oct 2014 Conference number: 3

Conference

Conference	3rd European Association of Veterinary Laboratory Diagnosticians
Number	3
Country/Territory	Italy
City	Pisa
Period	12/10/2014 → 15/10/2014

Keywords

Salmonella
ELISA
Poultry

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Refining the LPS-Antigen in Salmonella Antibody Elisa for Poultry Enhanced Specificity without Impairing Sensitivity. / Lauritsen, Klara Tølbøl; Lind, Peter; Klausen, Joan et al.
Abstract Book and Final Programme - 3rd European EAVLD Congress. Pisa, 2014. 143.

Research output: Chapter in Book/Report/Conference proceeding › Conference abstract in proceedings › Research › peer-review

TY - ABST

T1 - Refining the LPS-Antigen in Salmonella Antibody Elisa for Poultry Enhanced Specificity without Impairing Sensitivity

AU - Lauritsen, Klara Tølbøl

AU - Lind, Peter

AU - Klausen, Joan

AU - Andresen, Lars Ole

N1 - Conference code: 3

PY - 2014

Y1 - 2014

N2 - In the Danish serological surveillance for Salmonella in poultry (serum and egg yolk) a mix-ELISA is used, based on S. typhimurium and S. enteritidis antigens (Feld et al., 2000). When we evaluated results of the test retrospectively, over the years an unacceptably large fraction of seropositive findings could not be confirmed by the subsequent confirmatory bacteriological sampling in the herd. Therefore we tried to enhance specificity of the ELISA, without losing sensitivity, by refining the antigens used.

AB - In the Danish serological surveillance for Salmonella in poultry (serum and egg yolk) a mix-ELISA is used, based on S. typhimurium and S. enteritidis antigens (Feld et al., 2000). When we evaluated results of the test retrospectively, over the years an unacceptably large fraction of seropositive findings could not be confirmed by the subsequent confirmatory bacteriological sampling in the herd. Therefore we tried to enhance specificity of the ELISA, without losing sensitivity, by refining the antigens used.

KW - Salmonella

KW - ELISA

KW - Poultry

M3 - Conference abstract in proceedings

BT - Abstract Book and Final Programme - 3rd European EAVLD Congress

CY - Pisa

T2 - 3rd European Association of Veterinary Laboratory Diagnosticians

Y2 - 12 October 2014 through 15 October 2014

ER -

Refining the LPS-Antigen in Salmonella Antibody Elisa for Poultry Enhanced Specificity without Impairing Sensitivity

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