Reduction of Escherichia coli, Salmonella Enteritidis and Campylobacter jejuni in poultry manure by rearing of Musca domestica fly larvae

Steen Nordentoft, C. Fischer, L. Bjerrum, L. H. Heckmann, Birthe Hald

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Abstract

A major barrier for using animal waste as substrate for production of insects for feed or food is the concern for safety of the end products. In this study we investigated how rearing of fly larvae of Musca domestica in poultry manure influenced the counts of three pathogenic test strains (Escherichia coli, Salmonella Enteritidis and Campylobacter jejuni) and investigated whether these were transferred from manure to larvae, pupae or adults flies. We monitored quantitative microbiological changes over a study period of seven days. We showed that the decay in the numbers of E. coli, S. Enteritidis, and C. jejuni was faster in manure with rearing of fly larvae than in manure without larvae; an 8 log10 reduction of all three test bacteria was observed within four days in manure with larvae; compared to manure without larvae where a 1 to 2 log10 was observed. We found no sign of propagation of the pathogens in the larvae gut. All test strains were detectable in low numbers at day 4 in the larvae gut but only C. jejuni survived until day 7. In the pupae, only S. Enteritidis was detectable at day 3, and no test strains were found later than day 3. None of the test strain was isolated from adult flies. The total aerobic bacterial count remained at a high level throughout the study both in manure, in larvae and in pupae. In conclusion, our results showed that elimination of the pathogens from the manure was accelerated by larvae rearing, but were still detectable at low number in the larvae gut until end of the larvae stage. This study provides data for evaluation of feed safety of fly larvae reared on animal waste. Furthermore suggests a potential use for reduction of these pathogens in manure.
Original languageEnglish
JournalJournal of Insects as Food and Feed
Volume3
Issue number2
Pages (from-to)145-153
Number of pages9
ISSN2352-4588
DOIs
Publication statusPublished - 2017

Keywords

  • Feed safety
  • Insects
  • Microbial pathogens
  • Manure microbiology
  • Larvae composting

Cite this

Nordentoft, Steen ; Fischer, C. ; Bjerrum, L. ; Heckmann, L. H. ; Hald, Birthe. / Reduction of Escherichia coli, Salmonella Enteritidis and Campylobacter jejuni in poultry manure by rearing of Musca domestica fly larvae. In: Journal of Insects as Food and Feed. 2017 ; Vol. 3, No. 2. pp. 145-153.
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abstract = "A major barrier for using animal waste as substrate for production of insects for feed or food is the concern for safety of the end products. In this study we investigated how rearing of fly larvae of Musca domestica in poultry manure influenced the counts of three pathogenic test strains (Escherichia coli, Salmonella Enteritidis and Campylobacter jejuni) and investigated whether these were transferred from manure to larvae, pupae or adults flies. We monitored quantitative microbiological changes over a study period of seven days. We showed that the decay in the numbers of E. coli, S. Enteritidis, and C. jejuni was faster in manure with rearing of fly larvae than in manure without larvae; an 8 log10 reduction of all three test bacteria was observed within four days in manure with larvae; compared to manure without larvae where a 1 to 2 log10 was observed. We found no sign of propagation of the pathogens in the larvae gut. All test strains were detectable in low numbers at day 4 in the larvae gut but only C. jejuni survived until day 7. In the pupae, only S. Enteritidis was detectable at day 3, and no test strains were found later than day 3. None of the test strain was isolated from adult flies. The total aerobic bacterial count remained at a high level throughout the study both in manure, in larvae and in pupae. In conclusion, our results showed that elimination of the pathogens from the manure was accelerated by larvae rearing, but were still detectable at low number in the larvae gut until end of the larvae stage. This study provides data for evaluation of feed safety of fly larvae reared on animal waste. Furthermore suggests a potential use for reduction of these pathogens in manure.",
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Reduction of Escherichia coli, Salmonella Enteritidis and Campylobacter jejuni in poultry manure by rearing of Musca domestica fly larvae. / Nordentoft, Steen; Fischer, C.; Bjerrum, L.; Heckmann, L. H. ; Hald, Birthe.

In: Journal of Insects as Food and Feed, Vol. 3, No. 2, 2017, p. 145-153.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Reduction of Escherichia coli, Salmonella Enteritidis and Campylobacter jejuni in poultry manure by rearing of Musca domestica fly larvae

AU - Nordentoft, Steen

AU - Fischer, C.

AU - Bjerrum, L.

AU - Heckmann, L. H.

AU - Hald, Birthe

PY - 2017

Y1 - 2017

N2 - A major barrier for using animal waste as substrate for production of insects for feed or food is the concern for safety of the end products. In this study we investigated how rearing of fly larvae of Musca domestica in poultry manure influenced the counts of three pathogenic test strains (Escherichia coli, Salmonella Enteritidis and Campylobacter jejuni) and investigated whether these were transferred from manure to larvae, pupae or adults flies. We monitored quantitative microbiological changes over a study period of seven days. We showed that the decay in the numbers of E. coli, S. Enteritidis, and C. jejuni was faster in manure with rearing of fly larvae than in manure without larvae; an 8 log10 reduction of all three test bacteria was observed within four days in manure with larvae; compared to manure without larvae where a 1 to 2 log10 was observed. We found no sign of propagation of the pathogens in the larvae gut. All test strains were detectable in low numbers at day 4 in the larvae gut but only C. jejuni survived until day 7. In the pupae, only S. Enteritidis was detectable at day 3, and no test strains were found later than day 3. None of the test strain was isolated from adult flies. The total aerobic bacterial count remained at a high level throughout the study both in manure, in larvae and in pupae. In conclusion, our results showed that elimination of the pathogens from the manure was accelerated by larvae rearing, but were still detectable at low number in the larvae gut until end of the larvae stage. This study provides data for evaluation of feed safety of fly larvae reared on animal waste. Furthermore suggests a potential use for reduction of these pathogens in manure.

AB - A major barrier for using animal waste as substrate for production of insects for feed or food is the concern for safety of the end products. In this study we investigated how rearing of fly larvae of Musca domestica in poultry manure influenced the counts of three pathogenic test strains (Escherichia coli, Salmonella Enteritidis and Campylobacter jejuni) and investigated whether these were transferred from manure to larvae, pupae or adults flies. We monitored quantitative microbiological changes over a study period of seven days. We showed that the decay in the numbers of E. coli, S. Enteritidis, and C. jejuni was faster in manure with rearing of fly larvae than in manure without larvae; an 8 log10 reduction of all three test bacteria was observed within four days in manure with larvae; compared to manure without larvae where a 1 to 2 log10 was observed. We found no sign of propagation of the pathogens in the larvae gut. All test strains were detectable in low numbers at day 4 in the larvae gut but only C. jejuni survived until day 7. In the pupae, only S. Enteritidis was detectable at day 3, and no test strains were found later than day 3. None of the test strain was isolated from adult flies. The total aerobic bacterial count remained at a high level throughout the study both in manure, in larvae and in pupae. In conclusion, our results showed that elimination of the pathogens from the manure was accelerated by larvae rearing, but were still detectable at low number in the larvae gut until end of the larvae stage. This study provides data for evaluation of feed safety of fly larvae reared on animal waste. Furthermore suggests a potential use for reduction of these pathogens in manure.

KW - Feed safety

KW - Insects

KW - Microbial pathogens

KW - Manure microbiology

KW - Larvae composting

U2 - 10.3920/JIFF2016.0058

DO - 10.3920/JIFF2016.0058

M3 - Journal article

VL - 3

SP - 145

EP - 153

JO - Journal of Insects as Food and Feed

JF - Journal of Insects as Food and Feed

SN - 2352-4588

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ER -