Reduced ex vivo stimulated IL-6 response in infants randomized to fish oil from 9 to 18 months, especially among PPARG2 and COX2 wild types

Laurine B. S. Harslof, Camilla T. Damsgaard, Anders D. Andersen, Ditte L. Aakjaer, Kim F. Michaelsen, Lars I. Hellgren, Hanne Frøkiær, Ulla Vogel, Lotte Lauritzen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We investigated whether n-3 LCPUFA affected immune function in late infancy and explored effect-modification by single nucleotide polymorphisms (SNPs) and links to intestinal microbiota. Infants (n=105) were randomized to fish oil (FO, 1.2 g/d n-3 LCPUFA) or sunflower oil (SO)-supplements from age 9-18 months. Immune function was assessed by ex vivo cytokine production in stimulated blood and plasma immunoglobulin E (IgE). We genotyped functional SNPs in PPARG2 and COX2 and analyzed fecal microbiota by 16S-rRNA terminal restriction fragment length polymorphism. FO compared to SO reduced Lactobacillus paracasei-stimulated IL-6 at 18 months (P=0.03, n=104). This effect was most pronounced among infants wild-type for PPARG2-Pro12Ala and/or COX2-T8473C (P<0.05). Predominant bacterial fragments were associated with 18 months IgE in all infants (P=0.004) (bp100) and with IL-6 production among infants weaned before 9 months (P=0.047) (bp102). Thus, FO reduced IL-6 in a genotype-modified manner. The microbiota was partly linked to IL-6 and IgE, not directly to FO.
    Original languageEnglish
    JournalProstaglandins, Leukotrienes & Essential Fatty Acids
    Volume94
    Pages (from-to)21-27
    Number of pages7
    ISSN0952-3278
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Long-chain n-3 polyunsaturated fatty acids
    • Immune maturation
    • Child nutrition
    • Polymorphisms
    • Microbiota

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