Recording Microbial Signals In Soil: Developing Genetic Memory Devices For Detection Of Specialized Metabolites In Microbiomes

Morten Lindqvist Hansen*, Jens-Jakob Krogh Nørgaard, Lei Yang, Lars Jelsbak

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

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Abstract

Microbes reside in complex microbiomes, where secreted specialized metabolites play a major role in interspecies communication. However, investigating the role of specialized metabolites in microbial signaling is currently limited to methods that are disruptive and affected by detection limits. The aim of our research is to engineer soil-dwelling microbes capable of recording the presence of bioavailable specialized metabolites in situ in soil and rhizosphere microbiomes. This approach provides an ultrasensitive, non-disruptive alternative to existing methods, which additionally allow for both spatial and temporal investigations of signals in situ. The sensors function by inferring an irreversible genetic switch in the presence of the metabolite in question, which is catalyzed by a tightly controlled integrase. 

We have developed a handful of different sensors responding to pico-nanomolar concentrations of ecologically relevant metabolites. Each sensor has been tuned to avoid leaky expression in the absence of inducing metabolite in E. coli. Sensor sensitivity and responsiveness were analyzed with flow cytometry to generate response curves. Additionally, we have transferred our sensors to the soil- and rhizosphere compatible bacterium P. putida KT2440 and determined their effectiveness with flow cytometry. Finally, we have changed the output to genetic memory and verified the efficiency and orthogonality of the sensors with qPCR.

Whole-cell biosensors hold an immense potential to detect and record signals in natural environments. They are highly tunable genetic devices that serve as a promising alternative method for in situ investigations of natural products in complex environments such as soil- and plant microbiomes.
Original languageEnglish
Title of host publicationThe Danish Microbiological Society Annual Congress 2023 : Abstract book
Number of pages1
PublisherDanish Microbiological Society
Publication date2023
Pages71-71
Article number69
Publication statusPublished - 2023
EventThe Danish Microbiological Society Annual Congress 2023 - Copenhagen, Denmark
Duration: 13 Nov 202313 Nov 2023

Conference

ConferenceThe Danish Microbiological Society Annual Congress 2023
Country/TerritoryDenmark
CityCopenhagen
Period13/11/202313/11/2023

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