Receptor-mediated uptake of folic acid-functionalized dextran nanoparticles for applications in photodynamic therapy

Kathrin Butzbach, Matthias Konhäuser, Matthias Fach, Denise N. Bamberger, Benjamin Breitenbach, Bernd Epe, Peter R. Wich*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

In photodynamic therapy (PDT), photosensitizers and light are used to cause photochemically induced cell death. The selectivity and the effectiveness of the phototoxicity in cancer can be increased by a specific uptake of the photosensitizer into tumor cells. A promising target for this goal is the folic acid receptor α (FRα), which is overexpressed on the surface of many tumor cells and mediates an endocytotic uptake. Here, we describe a polysaccharide-based nanoparticle system suitable for targeted uptake and its photochemical and photobiological characterization. The photosensitizer 5, 10, 15, 20-tetraphenyl-21H, 23H-porphyrine (TPP) was encapsulated in spermine- and acetal-modified dextran (SpAcDex) nanoparticles and conjugated with folic acid (FA) on the surface [SpAcDex(TPP)-FA]. The particles are successfully taken up by human HeLa-KB cells, and a light-induced cytotoxicity is observable. An excess of free folate as the competitor for the FRα-mediated uptake inhibits the phototoxicity. In conclusion, folate-modified SpAcDex particles are a promising drug delivery system for a tumor cell targeted photodynamic therapy.
Original languageEnglish
Article number896
JournalPolymers
Volume11
Issue number5
Number of pages12
ISSN2073-4360
DOIs
Publication statusPublished - 2019

Keywords

  • Nanoparticles
  • Dextran
  • Targeting
  • Folic acid
  • Photodynamic therapy

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