Recent progress in heteronuclear long-range NMR of complex carbohydrates: 3D H2BC and clean HMBC

Sebastian Meier, Bent O. Petersen, Jens Øllgaard Duus, Ole W. Sørensen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The new NMR experiments 3D H2BC and clean HMBC are explored for challenging applications to a complex carbohydrate at natural abundance of 13C. The 3D H2BC experiment is crucial for sequential assignment as it yields heteronuclear one- and two-bond together with COSY correlations for the 1H spins, all in a single spectrum with good resolution and non-informative diagonal-type peaks suppressed. Clean HMBC is a remedy for the ubiquitous problem of strong coupling induced one-bond correlation artifacts in HMBC spectra of carbohydrates. Both experiments work well for one of the largest carbohydrates whose structure has been determined by NMR, not least due to the enhanced resolution offered by the third dimension in 3D H2BC and the improved spectral quality due to artifact suppression in clean HMBC. Hence these new experiments set the scene to take advantage of the sensitivity boost achieved by the latest generation of cold probes for NMR structure determination of even larger and more complex carbohydrates in solution.
Original languageEnglish
JournalCarbohydrate Research
Volume344
Issue number16
Pages (from-to)2274-2278
ISSN0008-6215
DOIs
Publication statusPublished - 2009

Keywords

  • 3D NMR
  • Heteronuclear NMR
  • HMBC
  • NMR assignment
  • H2BC

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