Recent insight into oligosaccharide uptake and metabolism in probiotic bacteria

Maher Abou Hachem, Joakim Mark Andersen, Rodolphe Barrangou, Marie Sofie Møller, Folmer Fredslund, Avishek Majumder, Morten Ejby Hansen, Sampo J. Lahtinen, Susanne Jacobsen, Leila Lo Leggio, Yong Jun Goh, Todd R. Klaenhammer, Birte Svensson

    Research output: Contribution to journalConference articleResearchpeer-review

    Abstract

    In recent years, a plethora of studies have demonstrated the paramount physiological importance of the gut microbiota on various aspects of human health and development. Particular focus has been set on probiotic members of this community, the best studied of which are assigned into the Lactobacillus and Bifidobacterium genera. Effects such as pathogen exclusion, alleviation of inflammation and allergies, colon cancer, and other bowel disorders are attributed to the activity of probiotic bacteria, which selectively ferment prebiotics comprising mainly non-digestible oligosaccharides. Thus, glycan metabolism is an important attribute of probiotic action and a factor influencing the composition of the gut microbiota. In the quest to understand the molecular mechanism of this selectivity for certain glycans, we have explored the routes of uptake and utilization of a variety of oligosaccharides differing in size, composition, and glycosidic linkages. A combination of "omics" technologies bioinformatics, enzymology and protein characterization proved fruitful in elucidating the protein transport and catabolic machinery conferring the utilization of glucosides, galactosides, and xylosides in the two clinically validated probiotic strains Lactobacillus acidophilus NCFM and Bifidobacterium animalis subsp. lactis Bl-04. Importantly, we have been able to identify and in some cases validate the specificity of several transport systems, which are otherwise poorly annotated. Further, we have demonstrated for the first time that non-naturally occurring tri- and tetra-saccharides are internalized and efficiently utilized by probiotic bacteria in some cases better than well-established natural prebiotics. Selected highlights of these data are presented, emphasising the importance and the diversity of oligosaccharide transport in probiotic bacteria. © 2013 Informa UK, Ltd.
    Original languageEnglish
    JournalBiocatalysis and Biotransformation
    Volume31
    Issue number4
    Pages (from-to)226-235
    ISSN1024-2422
    DOIs
    Publication statusPublished - 2013
    Event10th Carbohydrate Bioengineering Meeting - Prague, Czech Republic
    Duration: 21 Apr 201324 Apr 2013
    Conference number: 10

    Conference

    Conference10th Carbohydrate Bioengineering Meeting
    Number10
    CountryCzech Republic
    CityPrague
    Period21/04/201324/04/2013

    Keywords

    • Bacteria
    • Bioinformatics
    • Machinery
    • Metabolism
    • Physiology
    • Polysaccharides
    • Proteins
    • Oligosaccharides

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