Recent advances in the production of emulsifying peptides with the aid of proteomics and bioinformatics

Food industry aims to develop novel protein-based emulsifiers from sustainable sources (e.g. plants, seaweed/microalgae, microbial, insects) to satisfy the clean-label demand by consumers. Enzymatic hydrolysis releases peptides with enhanced surface properties compared to the parent alternative proteins. Traditionally, a trial-and-error top-down approach, which requires extensive costs in screening analyses, has been carried out to produce emulsifying peptides. This review presents the recent advances in a novel and fundamentally orthogonal bottom-up strategy, facilitated by quantitative proteomics and bioinformatic functional prediction, to produce emulsifying peptides by targeted enzymatic hydrolysis based on in silico proteolysis. Moreover, new insights on the relation between interfacial properties of peptides and emulsifying activity, as well as impact on stability of wet and dried emulsions, are discussed.