Abstract
Polyketides such as the antibiotic erythromycin or the immunosuppressant rapamycin, and non-ribosomal peptides, such as the antibiotics penicillin or vancomycin, are important classes of natural products. The core of these molecules are biosynthesized by large polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS), respectively. The modular architecture of these enzymatic assembly lines makes them interesting candidates for synthetic biology approaches. The re-engineering efforts aim to understand the molecular structure, produce new compounds, produce analogs of known compounds, tag the products or improve activity and/or yield. Here, we first consider the definition of PKS and NRPS modules, then give an overview of different strategies for re-engineering and finally review recent examples of PKS and NRPS reengineering.
Original language | English |
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Journal | Biotechnology and Bioprocess Engineering |
Volume | 25 |
Issue number | 6 |
Pages (from-to) | 886-894 |
ISSN | 1226-8372 |
DOIs | |
Publication status | Published - Dec 2020 |
Bibliographical note
Funding Information:The work of the authors is supported by grants of the Novo Nordisk Foundation [NNF10CC1016517, NNF16OC0021746]. The authors declare no conflict of interest.
Publisher Copyright:
© 2020, The Korean Society for Biotechnology and Bioengineering and Springer.
Keywords
- Antibiotics
- Non-ribosomal peptide
- NRPS
- PKS
- Polyketide
- Secondary metabolites
- Synthetic biology