Reassuringly low carriage of enteropathogens among healthy Swedish children in day care centres.

J. Kaarme, Rachel Hickman, T. Nevéus, J. Blomberg, C. Öhrmalm

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Infectious gastroenteritis is one of the most common diseases among children and has a considerable impact on health and socio-economy. Day care centres are high-risk environments for infections. The aim of this study was to investigate if asymptomatic preschool children constitute a reservoir for potential enteropathogens. In total, 438 individual diapers were collected from day care centres in Uppsala, Sweden, during spring and autumn, and molecular techniques were used to estimate the prevalence of asymptomatic carriage of multiple enteropathogens. Faecal samples were analysed with multiplex polymerase chain reaction (PCR) (xTAG® Gastrointestinal Pathogen Panel; Luminex Corporation, Toronto, Canada) targeting 21 different pathogens. Samples with a median fluorescence intensity above threshold were re-analysed with a second PCR assay. Sixteen of the 438 samples were positive for enteropathogens, 1.6% for enteric adenovirus, 0.7% for Campylobacter spp., and 0.7% for norovirus. Preschool children in Uppsala constitute a limited reservoir for potential enteropathogens.
Original languageEnglish
JournalPublic Health
Number of pages7
ISSN0033-3506
DOIs
Publication statusE-pub ahead of print - 2016
Externally publishedYes

Keywords

  • Enteropathogens
  • Carriage
  • Children
  • Preschool
  • Day care centres (DCCs)

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