Abstract
A novel real-time PCR microchip platform with integrated thermal system and polymer waveguides has been developed. By using the integrated optical system of the real-time PCR chip, cadF – a virulence gene of Campylobacter jejuni, could specifically be detected. Two different DNA binding dyes, SYTOX Orange and TO-PRO-3, were added to the PCR mixture to realize the real-time PCR. The presented approach shows reliable real-time quantitative information of the PCR amplification of the targeted gene.
Original language | English |
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Title of host publication | Proceedings of IEEE MEMS 2006 |
Publisher | IEEE |
Publication date | 2006 |
Pages | 542-545 |
ISBN (Print) | 0-7803-9475-5 |
DOIs | |
Publication status | Published - 2006 |
Event | 19th IEEE International Conference on Micro Electro Mechanical Systems - Istanbul, Turkey Duration: 22 Jan 2006 → 26 Jan 2006 Conference number: 19 http://ieeexplore.ieee.org/xpl/mostRecentIssue.jsp?punumber=10836 |
Conference
Conference | 19th IEEE International Conference on Micro Electro Mechanical Systems |
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Number | 19 |
Country/Territory | Turkey |
City | Istanbul |
Period | 22/01/2006 → 26/01/2006 |
Internet address |