Real-Time PCR using a PCR Microchip with Integrated Thermal System and Polymer Waveguides for the Detection of Campylobacter jejuni

Zhenyu Wang, Andrea Sekulovic, Jörg Peter Kutter, D.D. Bang, Anders Wolff

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    Abstract

    A novel real-time PCR microchip platform with integrated thermal system and polymer waveguides has been developed. By using the integrated optical system of the real-time PCR chip, cadF – a virulence gene of Campylobacter jejuni, could specifically be detected. Two different DNA binding dyes, SYTOX Orange and TO-PRO-3, were added to the PCR mixture to realize the real-time PCR. The presented approach shows reliable real-time quantitative information of the PCR amplification of the targeted gene.
    Original languageEnglish
    Title of host publicationProceedings of IEEE MEMS 2006
    PublisherIEEE
    Publication date2006
    Pages542-545
    ISBN (Print)0-7803-9475-5
    DOIs
    Publication statusPublished - 2006
    Event19th IEEE International Conference on Micro Electro Mechanical Systems - Istanbul, Turkey
    Duration: 22 Jan 200626 Jan 2006
    Conference number: 19
    http://ieeexplore.ieee.org/xpl/mostRecentIssue.jsp?punumber=10836

    Conference

    Conference19th IEEE International Conference on Micro Electro Mechanical Systems
    Number19
    Country/TerritoryTurkey
    CityIstanbul
    Period22/01/200626/01/2006
    Internet address

    Bibliographical note

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