Real-time PCR Detection of Food-borne Pathogenic Salmonella spp.

B. Malorny, D. Mäde, Charlotta Löfström

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Infections by Salmonella enterica are a significant public health concern worldwide. Salmonellae form a complex group of bacteria consisting of two species, six subspecies and more than 2500 serovars (serotypes). Mainly through ingestion of contaminated food or feed, they cause self-limiting gastrointestinal disease in a wide range of mammalian hosts. Within the last decade, numerous real-time PCR assays have been developed for rapid detection of salmonellae in potentially contaminated food or feed. Some of them were extensively validated and are useful for diagnostic laboratories. Furthermore, effective sample preparation prior to the analytical real-time PCR assay avoids inhibitory substances disturbing the PCR and contributes to a high sensitivity. We discuss appropriate sample preparation methods including enrichment procedures for various food items and analytical real-time PCR assays for the detection of Salmonella and give a state-of-the-art summary what targets are used and how valid the assays are to apply as diagnostic tool. Furthermore, recommendations for selection of an appropriate real-time PCR method are presented.
Original languageEnglish
Title of host publicationReal-Time PCR in Food Science: Current Technology and Applications
EditorsDavid Rodríguez-Lázaro
Number of pages21
PublisherCaister Academic Press
Publication date2013
Pages57-77
Chapter6
ISBN (Print)978-1-908230-15-7
Publication statusPublished - 2013

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