Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024

Leonard Schuele; Leandre Murhula Masirika; Jean Claude Udahemuka; Freddy Belesi Siangoli; Justin Bengehya Mbiribindi; Pacifique Ndishimye; Frank M. Aarestrup; Marion Koopmans; Bas B. Oude Munnink; Richard Molenkamp; GREATLIFE MPOX group

doi:10.2807/1560-7917.ES.2024.29.32.2400486

Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024

Leonard Schuele^*
, Leandre Murhula Masirika
, Jean Claude Udahemuka
, Freddy Belesi Siangoli
, Justin Bengehya Mbiribindi
, Pacifique Ndishimye
, Frank M. Aarestrup
, Marion Koopmans
, Bas B. Oude Munnink
, Richard Molenkamp
, GREATLIFE MPOX group

^*Corresponding author for this work

Erasmus University Rotterdam
University of Castilla-La Mancha
University of Rwanda
Division Provinciale de la Santé
African Institute for Mathematical Sciences

Research output: Contribution to journal › Letter › peer-review

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Abstract

Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.

Original language	English
Journal	Eurosurveillance
Volume	29
Issue number	32
Number of pages	6
ISSN	1025-496X
DOIs	https://doi.org/10.2807/1560-7917.ES.2024.29.32.2400486
Publication status	Published - 2024

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Schuele, L., Masirika, L. M., Udahemuka, J. C., Siangoli, F. B., Mbiribindi, J. B., Ndishimye, P., Aarestrup, F. M., Koopmans, M., Oude Munnink, B. B., Molenkamp, R., & GREATLIFE MPOX group (2024). Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024. Eurosurveillance, 29(32). https://doi.org/10.2807/1560-7917.ES.2024.29.32.2400486

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title = "Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024",

abstract = "Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.",

author = "Leonard Schuele and Masirika, \{Leandre Murhula\} and Udahemuka, \{Jean Claude\} and Siangoli, \{Freddy Belesi\} and Mbiribindi, \{Justin Bengehya\} and Pacifique Ndishimye and Aarestrup, \{Frank M.\} and Marion Koopmans and \{Oude Munnink\}, \{Bas B.\} and Richard Molenkamp and \{GREATLIFE MPOX group\} and Marjan Boter and Hayley Cassidy and Mambo, \{L{\'e}andre Mutimbwa\} and Gitta Overbeek and Musabyimana, \{Jean Pierre\} and Jules Ndoli and Saria Otani",

year = "2024",

doi = "10.2807/1560-7917.ES.2024.29.32.2400486",

language = "English",

volume = "29",

journal = "Eurosurveillance",

issn = "1025-496X",

publisher = "European Centre for Disease Prevention and Control (ECDC)",

number = "32",

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TY - JOUR

T1 - Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024

AU - Schuele, Leonard

AU - Masirika, Leandre Murhula

AU - Udahemuka, Jean Claude

AU - Siangoli, Freddy Belesi

AU - Mbiribindi, Justin Bengehya

AU - Ndishimye, Pacifique

AU - Aarestrup, Frank M.

AU - Koopmans, Marion

AU - Oude Munnink, Bas B.

AU - Molenkamp, Richard

AU - GREATLIFE MPOX group

AU - Otani, Saria

A2 - Boter, Marjan

A2 - Cassidy, Hayley

A2 - Mambo, Léandre Mutimbwa

A2 - Overbeek, Gitta

A2 - Musabyimana, Jean Pierre

A2 - Ndoli, Jules

PY - 2024

Y1 - 2024

N2 - Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.

AB - Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.

U2 - 10.2807/1560-7917.ES.2024.29.32.2400486

DO - 10.2807/1560-7917.ES.2024.29.32.2400486

M3 - Letter

C2 - 39119722

AN - SCOPUS:85201032574

SN - 1025-496X

VL - 29

JO - Eurosurveillance

JF - Eurosurveillance

IS - 32

ER -

Real-time PCR assay to detect the novel Clade Ib monkeypox virus, September 2023 to May 2024

Abstract

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