Real-time optical monitoring of cell culture in centrifugal microfluidics

Edwin En Te Hwu; Lina Gruzinskyte; Atsushi Ishimoto; Laura Serioli; Sriram Thoppe Rajendran; Akinobu Yamaguchi; Kinga Zor; Anja Boisen

Real-time optical monitoring of cell culture in centrifugal microfluidics

Edwin En Te Hwu^*, Lina Gruzinskyte, Atsushi Ishimoto, Laura Serioli, Sriram Thoppe Rajendran, Akinobu Yamaguchi, Kinga Zor, Anja Boisen

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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Abstract

We present a cellular-resolution (2 Pm) optical microscope that is rotating with a disc-shaped centrifugal microfluidics cell culture platform, inside an incubator. The microscope is powered wirelessly and is composed of a homemade optics and a wireless spy camera. The human cervical cancer cell line was, seeded and cultured on the disc, where their attachment and growth was monitored with the microscope. The microscope can wirelessly transmit microscopic images through the incubator to an external PC, which enables real-time (30 frames per second), continuous (without time limit) and remote (live Internet streaming) monitoring of the cell-based assays.

Original language	English
Title of host publication	MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Publication date	2020
Pages	1039-1040
ISBN (Electronic)	978-173341901-7
Publication status	Published - 2020
Event	24th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Online event Duration: 4 Oct 2020 → 9 Oct 2020 Conference number: 24 https://microtasconferences.org/microtas2020/

Conference

Conference	24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Number	24
Location	Online event
Period	04/10/2020 → 09/10/2020
Internet address	https://microtasconferences.org/microtas2020/

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@inproceedings{a387edc27b974d5aa07c059619e0662f,

title = "Real-time optical monitoring of cell culture in centrifugal microfluidics",

abstract = "We present a cellular-resolution (2 Pm) optical microscope that is rotating with a disc-shaped centrifugal microfluidics cell culture platform, inside an incubator. The microscope is powered wirelessly and is composed of a homemade optics and a wireless spy camera. The human cervical cancer cell line was, seeded and cultured on the disc, where their attachment and growth was monitored with the microscope. The microscope can wirelessly transmit microscopic images through the incubator to an external PC, which enables real-time (30 frames per second), continuous (without time limit) and remote (live Internet streaming) monitoring of the cell-based assays.",

author = "Hwu, {Edwin En Te} and Lina Gruzinskyte and Atsushi Ishimoto and Laura Serioli and {Thoppe Rajendran}, Sriram and Akinobu Yamaguchi and Kinga Zor and Anja Boisen",

year = "2020",

language = "English",

pages = "1039--1040",

booktitle = "MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

note = "24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 ; Conference date: 04-10-2020 Through 09-10-2020",

url = "https://microtasconferences.org/microtas2020/",

}

Hwu, EET , Gruzinskyte, L, Ishimoto, A, Serioli, L , Thoppe Rajendran, S, Yamaguchi, A, Zor, K & Boisen, A 2020, Real-time optical monitoring of cell culture in centrifugal microfluidics. in MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1039-1040, 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 04/10/2020.

Real-time optical monitoring of cell culture in centrifugal microfluidics. / Hwu, Edwin En Te ; Gruzinskyte, Lina; Ishimoto, Atsushi et al.

MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2020. p. 1039-1040.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

TY - GEN

T1 - Real-time optical monitoring of cell culture in centrifugal microfluidics

AU - Hwu, Edwin En Te

AU - Gruzinskyte, Lina

AU - Ishimoto, Atsushi

AU - Serioli, Laura

AU - Thoppe Rajendran, Sriram

AU - Yamaguchi, Akinobu

AU - Zor, Kinga

AU - Boisen, Anja

N1 - Conference code: 24

PY - 2020

Y1 - 2020

N2 - We present a cellular-resolution (2 Pm) optical microscope that is rotating with a disc-shaped centrifugal microfluidics cell culture platform, inside an incubator. The microscope is powered wirelessly and is composed of a homemade optics and a wireless spy camera. The human cervical cancer cell line was, seeded and cultured on the disc, where their attachment and growth was monitored with the microscope. The microscope can wirelessly transmit microscopic images through the incubator to an external PC, which enables real-time (30 frames per second), continuous (without time limit) and remote (live Internet streaming) monitoring of the cell-based assays.

AB - We present a cellular-resolution (2 Pm) optical microscope that is rotating with a disc-shaped centrifugal microfluidics cell culture platform, inside an incubator. The microscope is powered wirelessly and is composed of a homemade optics and a wireless spy camera. The human cervical cancer cell line was, seeded and cultured on the disc, where their attachment and growth was monitored with the microscope. The microscope can wirelessly transmit microscopic images through the incubator to an external PC, which enables real-time (30 frames per second), continuous (without time limit) and remote (live Internet streaming) monitoring of the cell-based assays.

M3 - Article in proceedings

SP - 1039

EP - 1040

BT - MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 4 October 2020 through 9 October 2020

ER -

Real-time optical monitoring of cell culture in centrifugal microfluidics

Abstract

Conference

UN SDGs

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