Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe

Cheng-Chih Hsu; Mariam S. ElNaggar; Yao Peng; Jinshu Fang; Laura M. Sanchez; Samantha J. Mascuch; Kirsten Amalie Møller; Emad K. Alazzeh; Jiri Pikula; Robert A. Quinn; Yi Zeng; Benjamin E. Wolfe; Rachel J. Dutton; Lena Gerwick; Lixin Zhang; Xueting Liu; Maria Månsson; Pieter C. Dorrestein

doi:10.1021/ac401613x

Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe

Cheng-Chih Hsu, Mariam S. ElNaggar, Yao Peng, Jinshu Fang, Laura M. Sanchez, Samantha J. Mascuch, Kirsten Amalie Møller, Emad K. Alazzeh, Jiri Pikula, Robert A. Quinn, Yi Zeng, Benjamin E. Wolfe, Rachel J. Dutton, Lena Gerwick, Lixin Zhang, Xueting Liu, Maria Månsson, Pieter C. Dorrestein

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Microorganisms such as bacteria and fungi produce a variety of specialized metabolites that are invaluable for agriculture, biological research, and drug discovery. However, the screening of microbial metabolic output is usually a time-intensive task. Here, we utilize a liquid microjunction surface sampling probe for electrospray ionization-mass spectrometry to extract and ionize metabolite mixtures directly from living microbial colonies grown on soft nutrient agar in Petri-dishes without any sample pretreatment. To demonstrate the robustness of the method, this technique was applied to observe the metabolic output of more than 30 microorganisms, including yeast, filamentous fungi, pathogens, and marine-derived bacteria, that were collected worldwide. Diverse natural products produced from different microbes, including Streptomyces coelicolor, Bacillus subtilis, and Pseudomonas aeruginosa are further characterized.

Original language	English
Journal	Analytical Chemistry
Volume	85
Issue number	15
Pages (from-to)	7014-7018
ISSN	0003-2700
DOIs	https://doi.org/10.1021/ac401613x
Publication status	Published - 2013

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Hsu, C.-C., ElNaggar, M. S., Peng, Y., Fang, J., Sanchez, L. M., Mascuch, S. J., Møller, K. A., Alazzeh, E. K., Pikula, J., Quinn, R. A., Zeng, Y., Wolfe, B. E., Dutton, R. J., Gerwick, L., Zhang, L., Liu, X., Månsson, M., & C. Dorrestein, P. (2013). Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe. Analytical Chemistry, 85(15), 7014-7018. https://doi.org/10.1021/ac401613x

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title = "Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe",

abstract = "Microorganisms such as bacteria and fungi produce a variety of specialized metabolites that are invaluable for agriculture, biological research, and drug discovery. However, the screening of microbial metabolic output is usually a time-intensive task. Here, we utilize a liquid microjunction surface sampling probe for electrospray ionization-mass spectrometry to extract and ionize metabolite mixtures directly from living microbial colonies grown on soft nutrient agar in Petri-dishes without any sample pretreatment. To demonstrate the robustness of the method, this technique was applied to observe the metabolic output of more than 30 microorganisms, including yeast, filamentous fungi, pathogens, and marine-derived bacteria, that were collected worldwide. Diverse natural products produced from different microbes, including Streptomyces coelicolor, Bacillus subtilis, and Pseudomonas aeruginosa are further characterized.",

author = "Cheng-Chih Hsu and ElNaggar, \{Mariam S.\} and Yao Peng and Jinshu Fang and Sanchez, \{Laura M.\} and Mascuch, \{Samantha J.\} and M{\o}ller, \{Kirsten Amalie\} and Alazzeh, \{Emad K.\} and Jiri Pikula and Quinn, \{Robert A.\} and Yi Zeng and Wolfe, \{Benjamin E.\} and Dutton, \{Rachel J.\} and Lena Gerwick and Lixin Zhang and Xueting Liu and Maria M{\aa}nsson and \{C. Dorrestein\}, Pieter",

year = "2013",

doi = "10.1021/ac401613x",

language = "English",

volume = "85",

pages = "7014--7018",

journal = "Analytical Chemistry",

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Hsu, C-C, ElNaggar, MS, Peng, Y, Fang, J, Sanchez, LM, Mascuch, SJ, Møller, KA, Alazzeh, EK, Pikula, J, Quinn, RA, Zeng, Y, Wolfe, BE, Dutton, RJ, Gerwick, L, Zhang, L, Liu, X, Månsson, M & C. Dorrestein, P 2013, 'Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe', Analytical Chemistry, vol. 85, no. 15, pp. 7014-7018. https://doi.org/10.1021/ac401613x

TY - JOUR

T1 - Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe

AU - Hsu, Cheng-Chih

AU - ElNaggar, Mariam S.

AU - Peng, Yao

AU - Fang, Jinshu

AU - Sanchez, Laura M.

AU - Mascuch, Samantha J.

AU - Møller, Kirsten Amalie

AU - Alazzeh, Emad K.

AU - Pikula, Jiri

AU - Quinn, Robert A.

AU - Zeng, Yi

AU - Wolfe, Benjamin E.

AU - Dutton, Rachel J.

AU - Gerwick, Lena

AU - Zhang, Lixin

AU - Liu, Xueting

AU - Månsson, Maria

AU - C. Dorrestein, Pieter

PY - 2013

Y1 - 2013

N2 - Microorganisms such as bacteria and fungi produce a variety of specialized metabolites that are invaluable for agriculture, biological research, and drug discovery. However, the screening of microbial metabolic output is usually a time-intensive task. Here, we utilize a liquid microjunction surface sampling probe for electrospray ionization-mass spectrometry to extract and ionize metabolite mixtures directly from living microbial colonies grown on soft nutrient agar in Petri-dishes without any sample pretreatment. To demonstrate the robustness of the method, this technique was applied to observe the metabolic output of more than 30 microorganisms, including yeast, filamentous fungi, pathogens, and marine-derived bacteria, that were collected worldwide. Diverse natural products produced from different microbes, including Streptomyces coelicolor, Bacillus subtilis, and Pseudomonas aeruginosa are further characterized.

AB - Microorganisms such as bacteria and fungi produce a variety of specialized metabolites that are invaluable for agriculture, biological research, and drug discovery. However, the screening of microbial metabolic output is usually a time-intensive task. Here, we utilize a liquid microjunction surface sampling probe for electrospray ionization-mass spectrometry to extract and ionize metabolite mixtures directly from living microbial colonies grown on soft nutrient agar in Petri-dishes without any sample pretreatment. To demonstrate the robustness of the method, this technique was applied to observe the metabolic output of more than 30 microorganisms, including yeast, filamentous fungi, pathogens, and marine-derived bacteria, that were collected worldwide. Diverse natural products produced from different microbes, including Streptomyces coelicolor, Bacillus subtilis, and Pseudomonas aeruginosa are further characterized.

U2 - 10.1021/ac401613x

DO - 10.1021/ac401613x

M3 - Journal article

C2 - 23819546

SN - 0003-2700

VL - 85

SP - 7014

EP - 7018

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 15

ER -

Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe

Abstract

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