Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe

Cheng-Chih Hsu, Mariam S. ElNaggar, Yao Peng, Jinshu Fang, Laura M. Sanchez, Samantha J. Mascuch, Kirsten Amalie Møller, Emad K. Alazzeh, Jiri Pikula, Robert A. Quinn, Yi Zeng, Benjamin E. Wolfe, Rachel J. Dutton, Lena Gerwick, Lixin Zhang, Xueting Liu, Maria Månsson, Pieter C. Dorrestein

    Research output: Contribution to journalJournal articleResearchpeer-review


    Microorganisms such as bacteria and fungi produce a variety of specialized metabolites that are invaluable for agriculture, biological research, and drug discovery. However, the screening of microbial metabolic output is usually a time-intensive task. Here, we utilize a liquid microjunction surface sampling probe for electrospray ionization-mass spectrometry to extract and ionize metabolite mixtures directly from living microbial colonies grown on soft nutrient agar in Petri-dishes without any sample pretreatment. To demonstrate the robustness of the method, this technique was applied to observe the metabolic output of more than 30 microorganisms, including yeast, filamentous fungi, pathogens, and marine-derived bacteria, that were collected worldwide. Diverse natural products produced from different microbes, including Streptomyces coelicolor, Bacillus subtilis, and Pseudomonas aeruginosa are further characterized.
    Original languageEnglish
    JournalAnalytical Chemistry
    Issue number15
    Pages (from-to)7014-7018
    Publication statusPublished - 2013

    Fingerprint Dive into the research topics of 'Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe'. Together they form a unique fingerprint.

    Cite this