The generalized phase contrast (GPC) method has been applied to transform a single TEM00 beam into a manifold of counterpropagating-beam traps capable of real-time interactive manipulation of multiple microparticles in three dimensions (3D). This paper reports on the use of low numerical aperture (NA), non-immersion, objective lenses in an implementation of the GPC-based 3D trapping system. Contrary to high-NA based optical tweezers, the GPC trapping system demonstrated here operates with long working distance (>10 mm), and offers a wider manipulation region and a larger field of view for imaging through each of the two opposing objective lenses. As a consequence of the large working distance, simultaneous monitoring of the trapped particles in a second orthogonal observation plane is demonstrated. (C) 2005 Optical Society of America.
Perch-Nielsen, I., Rodrigo, P. J., & Glückstad, J. (2005). Real-time interactive 3D manipulation of particles viewed in two orthogonal observation planes. Optics Express, 13(8), 2852-2857. https://doi.org/10.1364/OPEX.13.002852