Real-Time DNP NMR Observations of Acetic Acid Uptake, Intracellular Acidification, and of Consequences for Glycolysis and Alcoholic Fermentation in Yeast

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Abstract

Uptake and upshot in vivo: Straightforward methods that permit the real-time observation of organic acid influx, intracellular acidification, and concomitant effects on cellular-reaction networks are crucial for improved bioprocess monitoring and control (see scheme). Herein, dynamic nuclear polarization (DNP) NMR is used to observe acetate influx, ensuing intracellular acidification and the metabolic consequences on alcoholic fermentation and glycolysis in living cells.
Original languageEnglish
JournalChemistry: A European Journal
Volume19
Pages (from-to)13288 – 13293
ISSN0947-6539
DOIs
Publication statusPublished - 2013

Keywords

  • Acetate
  • Bioethanol
  • In vivo spectroscopy
  • NMR spectroscopy
  • Yeast fermentation

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