Rapid sample preparation for detection and identification of avian influenza virus from chicken faecal samples using magnetic bead microsystem

Raghuram Dhumpa, Minqiang Bu, Kurt Handberg, Anders Wolff, Dang Duong Bang

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Avian influenza virus (AIV) is an infectious agent of birds and mammals. AIV is causing huge economic loss and can be a threat to human health. Reverse transcriptase polymerase chain reaction (RT-PCR) has been used as a method for the detection and identification of AIV virus. Although RT-PCR is a sensitive method for detection of AIV, it requires sample preparation including separation and purification of AIV and concentrate viral RNA. It is laborious and complex process especially for diagnosis using faecal sample. In this study, magnetic beads were used for immunoseparation of AIV in chicken faecal sample by a magnetic microsystem. Using this system, all the 16 hemagglutinin (H) and 9 neuraminidase (N) subtypes of AIV were separated and detected in spiked faecal samples using RT-PCR, without an RNA extraction step. This rapid sample preparation method can be integrated with a total analysis microsystem and used for diagnosis of AIV.
    Original languageEnglish
    JournalJournal of Virological Methods
    Volume169
    Issue number1
    Pages (from-to)228-231
    ISSN0166-0934
    DOIs
    Publication statusPublished - 2010

    Keywords

    • Faecal sample
    • Sample preparation
    • Magnetic beads
    • Avian influenza virus
    • Magnetic microsystem

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