Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

Josiane P. Lafleur, Radoslaw Kwapiszewski, Thomas Glasdam Jensen, Jörg Peter Kutter

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The suitable optical properties of thiol–ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol–ene chips. This work focuses on thiol–ene substrates featuring an excess of thiol groups at their surface. The thiol–ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ! 17 SH nm"2. Biotin alkyne was patterned directly inside thiol–ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent waveinduced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications.
    Original languageEnglish
    JournalAnalyst
    Volume138
    Issue number3
    Pages (from-to)845-849
    ISSN0003-2654
    DOIs
    Publication statusPublished - 2013

    Keywords

    • Thiol-ene polymers
    • Biomolecule immobilization,
    • Surface functionalization
    • Biochips

    Cite this

    Lafleur, J. P., Kwapiszewski, R., Jensen, T. G., & Kutter, J. P. (2013). Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices. Analyst, 138(3), 845-849. https://doi.org/10.1039/c2an36424g